Applied Biosystems® Real-Time PCR Instruments
Celebrating Over 15 Years of Innovation in Real-Time PCRAs a leader in PCR innovation, Life Technologies offers you the gold standard in real-time PCR instrumentation. With our Applied Biosystems® real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with TaqMan® Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started and get the results you need to achieve your research goals. |
Which Real-Time PCR System Is Right for You?
| Easy to use for all levels of experience | Trusted performance & dye - versatility | Fast trusted performance & dye - versatility | High flexibility & intuitive design | Maximum throughput, flexibility and scalability | |||||
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| Reactions per Run | Up to 48/96 | Up to 96 | Up to 96 | Up to 384 | Up to 12000+ | ||||
| Block Format(s) | 48/96 | 96 | 96 Fast | 96, 96 Fast, 384, TaqMan Array (interchangeable) | 96, 96 Fast, 384, TaqMan Array, OpenArray® (interchangeable) |
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| Color Combinations (Multiplexing) | 3 and 4 | 5 | 5 | 21 | 21 | ||||
| Run Time | As fast as 40 min. | ~ 2 hrs | As fast as 30 min. | As fast as 30 min. | As fast as 30 min. | ||||
| Programmable Volumes | 1-40ul | 1 – 100 uL | 1-30ul | 1-200uL* | 1-200uL* | ||||
| Validated Volumes | 10-40ul | 20 – 100 uL | 5 – 30 uL | 1-100uL* | 33nl-100uL* | ||||
| Automation Compatible | No | No | No | Twister Robot Available | Twister Robot Available | ||||
| Remote Monitoring | Yes | No | No | Yes | Yes | ||||
| On-Board Memory | Yes – 1 Run | No | No | Yes – 100+ Runs | Yes – 100+ Runs | ||||
| Email Notification | No | No | No | Yes | Yes | ||||
| Calendar Scheduling | No | No | No | Yes | Yes | ||||
| Assist with 21 CFR p 11 compliance | Not available | Security, Auditing, eSignature packages available | |||||||
| Sensitivity | Down to 1 copy | ||||||||
| Dynamic Range | Up to 9 logs | ||||||||
| Chemistry | All real-time PCR based chemistries. Flexible to chemistries with or without passive reference dye. | ||||||||
| Applications Available | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, microRNA | Gene Expression, Genotyping, Copy Number Variation, Protein Thermal Shift, Protein Detection, microRNA | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, microRNA | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, microRNA | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, microRNA, digital PCR | ||||
| Validated Applications | N/A | Human Identification Testing | Food/Water, Pharmaceutical Analytics, Animal Health Testing | N/A | N/A | ||||
| IVD Platform | No | No | Available in certain countries | Available in certain countries | No | ||||
Instruments |
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| Temperature uniformity | ± 0.5°C within 30 seconds of clock start | ± 0.5°C within 30 seconds of clock start | ± 0.5°C within 30 seconds of clock start | ±0.5°C, within 30 sec of reaching temperature set point | ±0.5°C | ||||
Optics |
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| Excitation source | Blue LED | Tungsten-Halogen | Tungsten-Halogen | OptiFlex™ System with Halogen lamp | OptiFlex™ System with White LED light | ||||
| Filters/Colors | 4 filters factory calibrated for FAM™ / SYBR® Green, VIC® / JOE™, NED™ / TAMRA™ and ROX™ / dyes | 5 filter sets calibrated for FAM™ / SYBR® Green, VIC® / JOE™, NED™ / TAMRA™ / Cy3®, ROX™ / TEXAS RED, Cy5® dyes | 5 filter sets calibrated for FAM™ / SYBR® Green, VIC® / JOE™, NED™ / TAMRA™ / Cy3®, ROX™ / TEXAS RED, Cy5® dyes | 6 excitation filters (450–670 nm) 6 emission filters (500–720 nm) |
6 excitation filters (450–670 nm) 6 emission filters (500–720 nm) |
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| Detector | Photodiodes | CCD | CCD | CCD | CCD | ||||
Instrument specs |
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| Size | W 23.6 cm D 51.2 cm H 42.7 cm |
W 34 cm D 45 cm H 49 cm |
W 34 cm D 45 cm H 49 cm |
W 53.3 cm D 63.5 cm H 64.5 cm |
W 50.5 cm D 67.2 cm H 73.8 cm |
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*QuantStudio™ offers duplex capabilities with OpenArray® and multiplex capabilities for other formats.
** Up to 7 logs with OpenArray®.
Order Real-Time PCR InstrumentsContact a rep to request a quote for a real-time PCR system. Plastic Consumables GuideReaction tubes, 96-well and 384-well plates and adhesive films for real-time PCR. |
Other Real-Time PCR Instruments & Spare PartsFind the parts you need as well as part numbers for our legacy real-time PCR systems. Calibration & Verification KitsApplied Biosystems® Spectral Calibration kits and TaqMan® RNase P Instrument Verification Plates designed to help you calibrate and verify the performance of your real-time PCR system. |
Applied Biosystems® Thermal CyclersLooking for a traditional thermal cycler for end-point PCR? Instrument SoftwareA wide range of software applications to help you get the most from your Applied Biosystems® real-time PCR system. |
