Applied Biosystems® Real-Time PCR Instruments

Celebrating Over 15 Years of Innovation in Real-Time PCR
As a leader in PCR innovation, Life Technologies offers you the gold standard in real-time PCR instrumentation. With our Applied Biosystems® real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with TaqMan® Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started and get the results you need to achieve your research goals.

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Which Real-Time PCR System Is Right for You?

  Easy to use for all levels of experience   Trusted performance & dye - versatility   Fast trusted performance & dye - versatility   High flexibility & intuitive design   Maximum throughput, flexibility and scalability
 
 

  
 

  
 

  
 

  
 
Price $ / $$   $$   $$   $$$   $$$/$$$$
Speed        
Demonstrated Sensitivity 10 copies   10 copies   10 copies   Highest sensitivity, Down to 1 copy   Highest sensitivity, Down to 1 copy
Linear Dynamic Range 9 logs   5 logs guaranteed, 9 logs demonstrated   5 logs guaranteed, 9 logs demonstrated   Up to 9 logs   Up to 9 logs (96 & 384-well & TaqMan Array Card) Up to 7 logs (OpenArray)
Ease of Use        
Available Blocks 48 / 96-well   96-well   Fast 96-well   Standard & Fast 96-well
384-well & TaqMan® Array Card		   Highest throughput
Standard & Fast 96-well
384-well & TaqMan® Array Card
3,072 through-hole OpenArray® plate
Throughput /        

# of Colors		 3 / 4   5   5   21   21
Filters/Colors FAM™ / SYBR® Green, VIC® / JOE™ & ROX™ dyes
NED™ / TAMRA™ StepOnePlus only)		   FAM™ / SYBR® Green, VIC® / JOE™, NED™ / TAMRA™ / Cy3®, ROX™ / TEXAS RED, Cy5® dyes   FAM™ / SYBR® Green, VIC® / JOE™, NED™ / TAMRA™ / Cy3®, ROX™ / TEXAS RED, Cy5® dyes   6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)		   6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)
Excitation source Blue LED   Tungsten-Halogen   Tungsten-Halogen   OptiFlex™ System with Halogen lamp   OptiFlex™ System with White LED light
Detector Photodiodes   CCD   CCD   CCD   CCD
Size W 23.6 cm, D 51.2 cm, H 42.7 cm   W 34 cm, D 45 cm, H 49 cm   W 34 cm, D 45 cm, H 49 cm   W 53.3 cm, D 63.5 cm, H 64.5 cm   W 50.5 cm, D 67.2 cm, H 73.8 cm
Multiplex Quantitation Yes   Yes   Yes   Yes   Yes for 96-well/96-well Fast, 384-well, TaqMan array Card), Duplex for OpenArray®
Supported Chemistry SYBR® Green dye, TaqMan® probes. Capable of running other chemistries   SYBR® Green dye, TaqMan® probes. Capable of running other chemistries   TaqMan® Gene Expression & Quantitation Assays supported in Fast Mode; SYBR® Green dye, TaqMan® probes in other modes. Capable of running other chemistries   TaqMan® Gene Expression & Genotyping Assays, and SYBR® Green. Capable of running other chemistries   TaqMan® Gene Expression & Genotyping Assays, and SYBR® Green. Capable of running other chemistries


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Real-Time PCR Instrument Comparison

Plastic Consumables Guide

Other Real-Time PCR Instruments & Spare Parts

Calibration & Verification Kits

Applied Biosystems® Thermal Cyclers

Instrument Software