TaqMan® Mutation Detection Assays

Description

TaqMan® Mutation Detection Assays detect somatic mutations in genes that are associated with cancer. These research use only assays are powered by competitive allele-specific TaqMan® PCR technology, also known as castPCR™ technology. The castPCR™ technology is highly specific and sensitive, and it can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild type DNA.

High specificity—mutant allele detection is based on an allele-specific primer, while the wild-type background is suppressed by an MGB blocker oligonucleotide
High sensitivity—assays can detect as low as 0.1% mutant molecules in a background of wild type gDNA as demonstrated in spiking experiments
Wide dynamic range and excellent PCR efficiency—assays demonstrate at least 4 logs of dynamic range and an average efficiency of 100 ± 10%
Fast, simple workflow—like other TaqMan® Assays, typically requires 3 hours from sample to results, with minimum hands-on time
Our current assay set covers 778 assays to key mutation targets in 46 cancer genes

Catalog #	Product Name	Availability
4465804	TaqMan® Mutation Detection Assays (mutant allele assays and wild type allele assays)	Inventoried
4465805	TaqMan® EGFR Exon 19 Deletions Assay (mutant allele assay to 19 different EGFR exon 19 deletions)	Inventoried
4465807	TaqMan® Mutation Detection Reference Assays (gene reference assays)	Inventoried
4467538	TaqMan® Mutation Detection IPC Reagent Kit	Inventoried

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Specifications

General Specifications

Discrimination:	castPCR™ technology combines allele-specific TaqMan® qPCR with allele-specific MGB oligonucleotide blocker to effectively suppress non-specific amplification from the off-target allele.
Assay Components:	Each pre-formulated mutant or wild type allele assay contains: A 10X mix of unlabeled PCR primers, a TaqMan® MGB probe (FAM™ dye-labeled), and an MGB oligonucleotide blocker Each pre-formulated gene reference assay contains: A 10X mix of unlabeled PCR primers and a TaqMan® MGB probe (FAM™ dye-labeled)
Reactions:	Sufficient for 150 10µl reactions in a 384-well plate or 75 20µl reactions in a 96-well plate.
Expiration Date:	2 years from the date of manufacture
Assay Information File:	Includes sales order number, part number, assay ID, NCBI gene name, and entrez gene ID. Also includes Sanger COSMIC ID, nucleotide mutation, amino acid change, and mutation type.
Documentation :	Each assay shipment contains a CD-ROM disc containing: Protocol for TaqMan® Mutation Detection Assays (pdf file) TaqMan® Mutation Detection Assays Quick Reference Card (pdf file) Product Insert (pdf file) Assay Information File (XML and HTML formats) Understanding Your shipment document (pdf file) Safety data sheet (pdf file) Datasheet (pdf file) Certificate of Analysis (pdf file)
Tracking/Identification:	2D barcode laser-etched on the bottom of each assay tube 1D barcode printed on each rack of tubes