Mutation Detection Analysis Using TaqMan® Assays

Detect and measure somatic mutations in genes that are associated with cancer. These research use only assays are powered by competitive allele-specific TaqMan® PCR (castPCR™) technology. castPCR™ technology is highly specific and sensitive, and can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild type DNA. These assays are compatible with different sample types, such as cell lines, FFPE tissue samples, and fresh frozen tissue samples.
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Single Tube Assays

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Predesigned TaqMan® Mutation Detection Assays

Predesigned TaqMan® Assays

Our current assay set contains 285 assays to key mutation targets in 23 cancer genes. Mutant allele assays and corresponding gene reference assays are available to perform mutation detection experiments. Learn more about how the TaqMan® Mutation Detection Assays work.

TaqMan® Mutation Detection IPC Reagent Kit

 

The TaqMan® Mutation Detection IPC Reagent Kit is a set of optional internal positive control reagents that can be duplexed with any TaqMan® Mutation Detection Assay to provide a positive PCR control result. The TaqMan® Mutation Detection IPC Reagent Kit is pre-optimized for use with the TaqMan® Mutation Detection Assays.

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All Plated Formats

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96- or 384-well plates

 

Order your TaqMan® Mutation Detection Assays preplated into Fast or standard 96- or 384-well plates with the TaqMan® Custom Plating Service. Save time and reduce the resources needed for large-scale experiments.

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Data Analysis Software

 

For data analysis, Mutation Detector™ Software allows users to determine the mutation status of their samples from TaqMan® Mutation Detection Assay data collected on Applied Biosystems® Real-Time PCR Systems.

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QuantStudio 12K Flex Real-Time PCR System