Validation of Microarray Results Using TaqMan® Gene Expression Assays & Arrays
Microarrays are an excellent tool for initial target discovery, but not the best tool for evaluating differential gene expression. In addition, variability in microarray results exists from lab to lab, user to user, platform to platform, and every combination thereof. Additionally, the relatively small dynamic range of fluorescent microarrays (102 - 103) limits the technology with respect to sensitivity and specificity. It is therefore essential to use independent means to verify that genes of interest are differentially expressed, and to what extent.
TaqMan® Gene Expression Assays
TaqMan® Gene Expression Assays were used as the gold standard in the MicroArray Quality Control (MAQC) Project, which compared data from seven microarray platforms (Nature Biotechnology, September 2006). TaqMan® Gene Expression Assays can provide high specificity and sensitivity for their target, and can be used to evaluate a wide dynamic range of input target quantities.
Affordably priced, these predesigned real-time PCR assays minimize the cost and labor barriers historically associated with TaqMan® probe–based 5’ nuclease chemistry. You can receive the assays you need quickly, and the breadth of assays available makes accurate microarray validation possible.
To find the appropriate TaqMan Gene Expression assays, you can search using the microarray IDs that you are interested in validating.
TaqMan® Array Microfluidic Cards
The perfect tool for validating the tens or hundreds of hits from microarrays-TaqMan® Array Cards include 12 - 384 TaqMan® Gene Expression Assays in one easy-to-use 384-well card. TaqMan® Array Cards enable you to accomplish the validation necessary to arrive at the right answer easily and affordably. Choose from gene signature cards preformatted with TaqMan® Assays selected for specific pathways, biomarkers, or disease states, or customize a card with the assays of your choice.