TaqMan® MicroRNA Profiling Using the OpenArray® Real-Time PCR System

The OpenArray® Real-Time PCR System helps streamline miRNA profiling at a scale previously unattainable. Achieve the highest sample throughput for mid-density miRNA profiling—in a single day, one user can generate 27,000 data points on the OpenArray® Real-Time PCR System, with minimal pipetting steps and without the use of robotics.

Broad coverage - run 3 samples per OpenArray® Plate, using up to 818 TaqMan® Assays per sample (up to 758 unique assays)
High throughput - 9 samples per ~2.5 hr instrument run and 36 samples per 8 hr working day
Cost effective - rapid sample screening with minimal hands-on time
Minimal sample requirement - only 200 ng of total RNA needed for a full miRNA profile

TaqMan® OpenArray® MicroRNA Panel Workflow

Applied Biosystems offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

Recommended Products

TaqMan® miRNA ABC Purification Kit
Purify high-quality miRNA from difficult sample types without hazardous chemicals. This kit enables single-tube isolation of specific miRNA from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes.

TaqMan® miRNA ABC Purification Kit

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods.

Designed to streamline miRNA profiling, Megaplex™ RT Primers are pools of RT primers identical to those in individual TaqMan® MicroRNA Assays. These primers are ideal for global miRNA expression profiling; just two pools provide comprehensive coverage of the Sanger miRBase v14 and v15 database content for human and rodent, respectively. The content of each pool matches corresponding TaqMan® OpenArray® Human or Rodent MicroRNA Panels. The TaqMan® MicroRNA RT Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used to convert miRNA to cDNA prior to real-time PCR quantitation.

Recommended Products

Megaplex™ RT Primers
Whether your profiling experiment requires ultimate sensitivity, broad coverage or both, Megaplex™ Primer Pools provide the flexibility to reach your research goals. Megaplex™ Primer Pools provide comprehensive coverage of Sanger miRBase v14 and v15 (for human and rodent, respectively), and when used with TaqMan® MicroRNA Arrays, offer an ideal miRNA profiling solution.

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

Megaplex™ PreAmp Primers generate a comprehensive miRNA expression profile using as little as 1 ng of input total RNA. With content matched to specific Megaplex™ RT Primers and TaqMan® OpenArray® Human MicroRNA Panels, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to uniformly amplify all miRNAs present in the original sample significantly increasing assay sensitivity.

Recommended Products

Megaplex™ PreAmp Primers
For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA.

TaqMan® PreAmp Master Mix
TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

TaqMan® PreAmp Master Mix

Load sample mixes onto TaqMan® OpenArray® MicroRNA Panels using the OpenArray® AccuFill™ System. Then insert the TaqMan® OpenArray® MicroRNA Panel into the OpenArray® case filled with immersion fluid and seal with glue. Perform a real-time plate read using the OpenArray® Real-Time PCR Instrument.

Recommended Products

OpenArray® Real-Time PCR Platform
The OpenArray® Real-Time PCR Instrument and OpenArray® AccuFill™ System combination can process hundreds to thousands of samples per day, enabling mid-density, high-throughput results with ease of use and without robotics.

TaqMan® OpenArray® MicroRNA Panels
TaqMan® OpenArray® MicroRNA Panels contain preconfigured sets of TaqMan® MicroRNA Assays for human profiling studies in the highest throughput format available from Life Technologies. The human panel lets you run 3 samples and a total of 2,274 unique TaqMan® MicroRNA Assays at once.

TaqMan® OpenArray® Real-Time PCR Master Mix
TaqMan® OpenArray® Real-Time PCR Master Mix is conveniently formulated to support microRNA (miRNA) profiling using TaqMan® OpenArray® MicroRNA Panels on the OpenArray® Real-Time PCR System.

Recommended Products

RealTime StatMiner® Software
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.

Publications and Literature

Product Bulletin: OpenArray® Real-Time PCR System

Product Bulletin: TaqMan® MicroRNA Assays and Arrays

Specification Sheet: OpenArray® Real-Time PCR Platform