TaqMan® MicroRNA Profiling Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods.

• mirVana™ miRNA Isolation Kit
• mirVana™ miRNA Isolation Kit (without Phenol)

Megaplex™ RT Primers
Whether your profiling experiment requires ultimate sensitivity, broad coverage, or both, Megaplex™ Primer Pools provide the flexibility to reach your research goals. Megaplex™ Primer Pools provide comprehensive coverage of miRNAs in the Sanger miRBase database, and when used with TaqMan® MicroRNA Arrays, offer an ideal miRNA profiling solution.

• Megaplex™ Primer Pools, Human Pools Set v3.0
• Megaplex™ Primer Pools, Rodent Pools Set v3.0

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

• TaqMan® MicroRNA Reverse Transcription Kit, 200 reactions
• TaqMan® MicroRNA Reverse Transcription Kit, 1000 reactions

Megaplex™ PreAmp Primers
For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect miRNAs expressed at low levels, enabling the generation of a comprehensive expression profile using very little sample—as little as 1 ng of input total RNA.

• Megaplex™ Primer Pools, Human Pools Set v3.0
• Megaplex™ Primer Pools, Rodent Pools Set v3.0

TaqMan® PreAmp Master Mix
TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions. The TaqMan® PreAmp Master Mix Kit consists of TaqMan® PreAmp Master Mix and TaqMan® Gene Expression Master Mix.

• TaqMan® PreAmp Master Mix Kit
• TaqMan® PreAmp Master Mix

TaqMan® OpenArray® MicroRNA Panels 
TaqMan® OpenArray® MicroRNA Panels contain preconfigured sets of TaqMan® MicroRNA Assays, for either human or rodent profiling studies, in the highest- throughput format available. With these panels, you can run 3 samples simultaneously, each with over 700 unique TaqMan® MicroRNA Assays.

• TaqMan® OpenArray® Human MicroRNA Panel QuantStudio™ 12K Flex
• TaqMan® OpenArray® Rodent MicroRNA Panel QuantStudio™ 12K Flex

TaqMan® OpenArray® Real-Time PCR Master Mix
TaqMan® OpenArray® Real-Time PCR Master Mix is conveniently formulated to support miRNA profiling using TaqMan® OpenArray® MicroRNA Panels on the QuantStudio™ 12K Flex Real-Time PCR System.

• TaqMan® OpenArray® Real-Time PCR Master Mix, 1.5 mL
• TaqMan® OpenArray® Real-Time PCR Master Mix, 5 mL

OpenArray® AccuFill™ System

• QuantStudio™ 12K Flex OpenArray® AccuFill™ System

The OpenArray® AccuFill™ System loads the sample mix from a 384‐well plate onto the TaqMan® OpenArray® Real-Time PCR plate with minimal human intervention.

• OpenArray® Sample Tracker Software

OpenArray® Sample Tracker Software integrates the sample information from a 384‐well plate file with the assay information contained in the plate setup file. 

• OpenArray® AccuFill™ System Tips
• OpenArray® AccuFill™ System Tips, 10 pack

QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block
The QuantStudio™ 12K Flex Real-time PCR Systemand QuantStudio™ 12K Flex OpenArray® AccuFill™ System combination can process hundreds to thousands of samples per day, enabling mid-density, high-throughput results with ease of use and without robotics.

RealTime StatMiner® Software
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.