TaqMan® Pri-miRNA Assays

TaqMan® Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold standard assay performance and data quality for pri-miRNA studies.

• Highly specific – quantitiate miRNA transcription from a single genomic locus
• Fast, simple, and scalable – two-step RT-qPCR provides high-quality results
• A new perspective – measure miRNA expression at the gene level

TaqMan® Pri-miRNA Assays offer the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan® Genomic Assays are renowned, to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan® Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and Selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan® Pri-miRNA Assay and TaqMan® MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.