MicroRNA Analysis Using TaqMan® Assays

TaqMan® MicroRNA Assays are innovative tools for miRNA research—from isolation through discovery, profiling, quantitation, validation, and functional analysis.

TaqMan® MicroRNA Assays employ a novel target-specific stem-loop reverse transcription primer to address the challenge of the short length of mature miRNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay–based real-time PCR. Also, the stem-loop structure in the tail of the primer confers a key advantage to these assays: specific detection of the mature, biologically active miRNA.
Use our Search Tool below to find your assays.

miRBase v.19 update: Our content is now aligned with release 19 of the miRBase database and provides comprehensive coverage for each of the 193 listed species.

Download: Complete list of TaqMan® MicroRNA Assays

Download: Protocol for multiplexing RT and preamplification reactions with TaqMan® MicroRNA Assays

Please note:
For miRNAs not included in miRBase and for any other Small RNAs, we offer custom designed Custom TaqMan® Small RNA Assay.
For noncoding human, mouse, or rat transcripts longer than 200 nucleotides, we offer TaqMan® Non-coding RNA Assays.

Use our Search Tool below to find the single tube assays you need.

Assay Search Tool:


TaqMan® Pri-miRNA Assays TaqMan® Pri-miRNA Assays for detecting and measuring primary miRNAs from human, mouse, or rat species. Learn More		TaqMan® Non-coding RNA Assays TaqMan® Non-coding RNA Assays for reliable detection and quantitation of noncoding human, mouse, or rat transcripts longer than 200 nucleotides. Learn More

Custom TaqMan® Small RNA Assays
Custom assays to quantitate small RNA are ideal to quantitate your small RNA when a predesigned assay is unavailable—all you need is the sequence of interest.	Learn More

Plated Formats


96-Well Plates Order your TaqMan® MicroRNA Assays preplated into Fast or standard 96-well plates with the TaqMan® Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets. Learn More 384-Well Microfluidic Cards 384-well cards are ideal for profiling studies across a small number of samples (up to 8 samples per card) or with limited sample material (each reaction requires only 2 µL of sample) and are compatible with Applied Biosystems® ViiA™ 7 and 7900HT Real-Time PCR Systems. Learn More		384-Well Plates Order your TaqMan® MicroRNA Assays preplated into Fast or standard 384-well plates with the TaqMan® Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets. Learn More OpenArray® Plates These plates accommodate 3,072 reactions at once, delivering the high-throughput capabilities required for large profiling studies. Designed for use with the Applied Biosystems® OpenArray® Real-Time PCR System and the new QuantStudio™ 12K Flex Real-Time PCR System. Learn More