Somatic Mutation Detection Workflow
All TaqMan® Mutation Detection Assays have undergone extensive testing to ensure high sensitivity and specificity. The first set of released assays, covering 14 KRAS, 29 EGFR, and the BRAF V600E mutations, underwent additional testing, including determination of (a) the difference in inherent amplification efficiency between mutant allele assays and corresponding reference assays, to enable quantitative analysis of percent mutation in a sample, and (b) assay detection ΔCt cutoff values using spiked cell line gDNA samples.
For additional information, please see our product information page.
For research use only. Not for use in diagnostic procedures.