Small RNA Analysis
How It Works
Custom TaqMan® Small RNA Assays employ an innovative target-specific stem-loop reverse transcription primer to address the challenge of the short length of the small RNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay-based qPCR. Also, the stem-loop structure in the tail of the primer confers a key advantage if you are studying miRNAs: specific detection of the mature, biologically active miRNA.
Small RNA Analysis Experimental Workflow
Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs. Each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).
mirVana™ miRNA Isolation Kit
RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
TaqMan® MicroRNA Cells-to-CT™ Kit