miRNA Quantitation

Workflow Features

Ideal for quantitating individual miRNAs using Real-Time PCR from a broad assortment of species, including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis
Each kit includes the TaqMan® MicroRNA Assay and reverse transcription primer specific for the mature miRNA target of interest
Assays are continuously added for human, mouse, and rat species to stay aligned with the Sanger miRBase Registry

How It Works

TaqMan® MicroRNA Assays employ an innovative target-specific stem-loop reverse transcription primer to address the challenge of the short length of mature miRNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay-based real-time PCR. Also, the stem-loop structure in the tail of the primer confers a key advantage to these assays: specific detection of the mature, biologically active miRNA.

miRNA Quantitation Experimental Workflow

Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

Recommended Products

		TaqMan® miRNA ABC Purification Kit Purify high-quality miRNA from difficult sample types without hazardous chemicals. This kit enables single-tube isolation of specific miRNA from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes. TaqMan® miRNA ABC Purification Kit
		mirVana™ miRNA Isolation Kit Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs. mirVana™ miRNA Isolation Kit mirVana™ miRNA Isolation Kit (without Phenol)
		RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses. RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
		TaqMan® MicroRNA Cells-to-CT™ Kit Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification. TaqMan® MicroRNA Cells-to-CT™ Kit TaqMan® MicroRNA Cells-to-CT™ Kit w/Manual Cells-to-CT Bulk Lysis Reagents Cells-to-CT™ Stop Solution

Two-step TaqMan® MicroRNA Assays, containing the TaqMan® Assay and reverse transcription primer specific for the target of interest, are designed for individual miRNA targets. The TaqMan® MicroRNA Reverese Transcription Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used with a single RT primer provided with each individual TaqMan® MicroRNA Assays or with Megaplex™ RT Primers to convert miRNA to cDNA prior to real-time PCR quantitation.

Recommended Products

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

TaqMan® MicroRNA Assays (RT tube)
TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. The assays target only mature miRNAs, not their precursors, ensuring biologically relevant results.

For the final quantitation step, the cDNA product generated in step 2 is mixed with TaqMan® Universal Master Mix II and added to the matched TaqMan® MicroRNA Assay.

Recommended Products

		TaqMan® MicroRNA Assays (individual assays) TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. The assays target only mature miRNAs, not their precursors, ensuring biologically relevant results.
		TaqMan® Universal Master Mix II TaqMan® Universal Master Mix II offers convenience and reliability for all real-time applications including microRNA, gene expression, and genotyping studies.

Integromics RealTime StatMiner® Software is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. The software easily imports raw data or Ct values from any Applied Biosystems® Real-Time PCR System and guides you though every step of analysis for accurate results in minutes.

Recommended Products

RealTime StatMiner® Software (optional)
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you through every step of analysis, giving you accurate results in minutes.