How It Works
TaqMan® MicroRNA Assays employ an innovative target-specific stem-loop reverse transcription primer to address the challenge of the short length of mature miRNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay-based real-time PCR. Also, the stem-loop structure in the tail of the primer confers a key advantage to these assays: specific detection of the mature, biologically active miRNA.
miRNA Quantitation Experimental Workflow
Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).
TaqMan® miRNA ABC Purification Kit
mirVana™ miRNA Isolation Kit
RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
TaqMan® MicroRNA Cells-to-CT™ Kit
For research use only. Not intended for any animal or human therapeutic or diagnostic use.