Long noncoding RNA (ncRNA) Analysis Using Real-Time PCR

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs.

• mirVana™ miRNA Isolation Kit
• mirVana™ miRNA Isolation Kit (without Phenol)

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

• RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

TURBO DNA-free™ Kit
TURBO™ DNase is a recombinant, engineered form of DNase I that is more efficient than wild-type DNase I at digesting trace amounts of unwanted DNA in RNA samples. The TURBO DNA-free™ Kit comes with all reagents needed to perform 50 TURBO™ DNase treatments plus a highly effective DNase Inactivation Reagent, making this kit the tool of choice for RT-PCR sample preparation.

• TURBO DNA-free™
• TURBO DNA-free™ w/Manual

TaqMan® MicroRNA Cells-to-CT™ Kit
Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification.

• TaqMan® MicroRNA Cells-to-CT™ Kit
• TaqMan® MicroRNA Cells-to-CT™ Kit w/Manual
• Cells-to-CT Bulk Lysis Reagents
• Cells-to-CT™ Stop Solution

High Capacity RNA-to-cDNA Kit
The High Capacity RNA-to-cDNA™ Kit provides high quality reverse transcription that delivers a streamlined workflow with maximum sensitivity and dynamic range.

• High Capacity RNA-to-cDNA Kit
• Protocol: High Capacity RNA-to-cDNA Kit
• Quick Reference Card: High Capacity RNA-to-cDNA Kit

TaqMan® PreAmp Master Mix
TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

• TaqMan® PreAmp Master Mix Kit
• TaqMan® PreAmp Master Mix

TaqMan® Non-coding RNA Assays
TaqMan® Non-coding RNA Assays provide a highly reliable way to measure expression of long non-coding transcripts. These assays are based on the same gold standard TaqMan® Assays technology that is used in the TaqMan® Gene Expression Assays collection. With TaqMan® Assay technology, you get the high specificity and sensitivity you need for quantification and validation studies.

TaqMan® Universal Master Mix II
TaqMan® Universal Master Mix II offers convenience and reliability for all real-time applications including microRNA, gene expression, and genotyping studies.

RealTime StatMiner® Software (optional)
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you through every step of analysis, giving you accurate results in minutes.