SNP Genotyping With Real-Time PCR

SNP Genotyping Using 48-, 96-, and 384-Well Real-Time PCR Instruments

TaqMan® SNP Genotyping Assays use TaqMan® 5´ nuclease assay chemistry for amplifying and detecting specific SNP alleles in purified genomic DNA samples. Each assay allows researchers to genotype individuals for a specific SNP.

World’s largest collection of single-tube, ready-to-use SNP assays available
Robust custom assay design pipeline enables highly accurate, reproducible and reliable results
Easy ordering and a simple workflow enable quick results
Suggested panels to get you started quickly for many applications
Superior Applied Biosystems® real-time PCR systems available to meet all throughput needs and budgets for processing and analyzing <1,000 samples a day

Experimental Workflow for SNP Genotyping

Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

Products

TaqMan® Sample-to-SNP™ Kit
The TaqMan® Sample-to-SNP™ Kit provides a streamlined protocol for performing TaqMan® chemistry-based genotyping analysis from any sample with a single kit.

BloodPrep® DNA Chemistry for Cultured Cells and Blood
BloodPrep® DNA Chemistry is designed to rapidly isolate DNA from fresh or frozen blood from any animal species, tissue-cultured cells, or buccal swabs. The protocol is designed for use with the Applied Biosystems® 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

Select or design TaqMan® probes.

Life Technolgoies offers a variety of TaqMan® Genotyping Assays for a number of real-time PCR analysis applications.

Recommended Products

TaqMan® SNP Genotyping Assays
With 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70,000 cSNPs and 160,000 validated assays, TaqMan® SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan® reagent-based chemistry.

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Custom TaqMan® SNP Genotyping Assays
Order custom, single-tube TaqMan® reagent-based assays to perform genotyping studies with any possible SNP in any organism. The Custom TaqMan® Assays Design Tool allows you to quickly order Custom TaqMan® Assays by submitting target sequences of interest or custom primer and probe sequences from any organism.

design assays

TaqMan® Drug Metabolism Assays
Genotype 2,700 high value polymorphisms located in regulatory elements and coding regions for 221 drug metabolism and transporter genes.

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Prepare reaction plate and amplify DNA using sequence specific primers and TaqMan® probe on an Applied Biosystems® real-time PCR system or thermal cycler.

Reagents & Kits

		TaqMan® Genotyping Master Mix Obtain unrivaled cluster resolution for unambiguous SNP allelic discrimination. Optimized for end-point fluorescence detection in genotyping applications. TaqMan® GTXpress™ Master Mix, (2 x 50 mL) TaqMan® Genotyping Master Mix Protocol TaqMan® Genotyping Master Mix, Mini-Pack (1mL) TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL) TaqMan® Genotyping Master Mix, 1 Bulk Pack (1 x 50 mL) TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL) TaqMan® Genotyping Master Mix, Multi-Bulk Pack (2 x 50 mL)
Instruments & Accessories
		Veriti® 96-Well Thermal Cycler The Veriti® 96-Well Thermal Cycler delivers excellent PCR and the proven reliability of Applied Biosystems 9600 and 9700 GeneAmp® PCR instruments. With the added control of VeriFlex™ Blocks, you have six independent temperature blocks providing precise control over your PCR optimization. Veriti® 96-Well Thermal Cycler Veriti® Thermal Cycler, IQ User-Performed Test Procedures Veriti® Thermal Cycler, OQ/IPV User-Performed Test Procedures Veriti® Thermal Cycler, IQ/OQ User-Performed Test Procedures Veriti® Thermal Cycler, 1-year Extended Warranty Veriti® Thermal Cycler, 3-year Extended Warranty

		Dual Flat Block GeneAmp® PCR System 9700 GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The System consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with TaqMan® OpenArray® Genotyping Plates on the OpenArray Real-Time PCR System. GeneAmp® PCR System 9700 and 9800 Fast Thermal Cycler, IQ User-Performed Test Procedures GeneAmp® PCR System 9700 and 9800 Fast Thermal Cycler, OQ/IPV User-Performed Test Procedures Dual Flat Block GeneAmp® PCR System 9700 Sample Module only OpenArray® Plate Adaptor for Dual Flat Block GeneAmp® PCR System 9700 OpenArray® Plate Compression Pad for Dual Flat Block GeneAmp® PCR System 9700 Dual Flat Block GeneAmp® PCR System 9700 GeneAmp® PCR System 9700 and 9800 Fast Thermal Cycler, IQ/OQ User-Performed Test Procedures GeneAmp® PCR System 9700, Base Module Dual Flat Block GeneAmp® PCR System 9700, 3-year Extended Warranty Dual Flat Block GeneAmp® PCR System 9700, 1-year Extended Warranty

		StepOnePlus™ Real-Time PCR System The StepOnePlus™ Real-Time PCR System makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels. StepOnePlus™ Real-Time PCR System with Laptop StepOnePlus™ Real-Time PCR System with Tower StepOnePlus™ Real-Time PCR System StepOnePlus™ Real-Time PCR System, 1-year Extended Warranty StepOnePlus™ Real-Time PCR System, 3-year Extended Warranty

		StepOne™ Real-Time PCR System Applied Biosystems’ 48-well low-throughput real-time PCR instrument makes it simple and easy to get publication ready high quality data on day one. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR. StepOnePlus™ Real-Time PCR System with Laptop StepOnePlus™ Real-Time PCR System with Tower StepOnePlus™ Real-Time PCR System StepOnePlus™ Real-Time PCR System, 1-year Extended Warranty StepOnePlus™ Real-Time PCR System, 3-year Extended Warranty

		7500 Fast Real-Time PCR System The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. 7500 Fast Real-Time PCR System with Dell™ Notebook 7500 Fast Real-Time PCR System with Dell™Tower Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems 7500 Fast System Upgrade Kit 7500 Fast System SDS v1.4 21 CFR Part 11 Module High Resolution Melt (HRM) Software v2.0

		7500 Real-Time PCR System The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes. 7500 Real-Time PCR System with Dell™ Notebook 7500 Real-Time PCR System with Dell™Tower Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems 7500 Fast System Upgrade Kit 7500 System SDS v1.4 21 CFR Part 11 Module

		7900HT Fast Real-Time PCR System The 7900HT Fast Real-Time PCR System combines 384-well plate compatibility with fully automated robotic loading—and now also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments. ABI PRISM® 7900HT Sequence Detection System User's Manual 7900HT Fast Real-Time PCR System with 384-Well Block Module 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module 7900HT System Automation Accessory Upgrade 7900HT System Standard 96-Well Block Upgrade Kit 7900HT System Standard 384-Well Block Upgrade Kit 7900HT System Fast 96-Well Block Upgrade Kit 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module 7900HT Fast Real-Time Service Upgrade 7900HT Fast Real-Time Service Upgrade with Computer

		ViiA™ 7 Real-Time PCR System The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab. Applied Biosystems® ViiA™ 7 Real-Time PCR System with 384-Well Block Applied Biosystems® ViiA™ 7 Real-Time PCR System with TaqMan® Array Applied Biosystems® ViiA™ 7 Real-Time PCR System with 96-Well Fast Block Applied Biosystems® ViiA™ 7 Real-Time PCR System with 96-Well Block ViiA™ 7 System Automation Accessory Robot (100–240 V) 384-Well Block Upgrade Kit TaqMan® Array Upgrade Kit 96-Well Fast Block Upgrade Kit 96-Well Block Upgrade Kit HRM Software Module for ViiA™ 7 System 21 CFR Part 11 Software Module for ViiA™ 7 System

Perform an endpoint plate read using Applied Biosystems® real-time PCR systems; TaqMan® SNP Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.

Instruments & Accessories

		StepOnePlus™ Real-Time PCR System The StepOnePlus™ Real-Time PCR System makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96-well, 4 color system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels. StepOnePlus™ Real-Time PCR System with Laptop StepOnePlus™ Real-Time PCR System with Tower StepOnePlus™ Real-Time PCR System StepOnePlus™ Real-Time PCR System, 1-year Extended Warranty StepOnePlus™ Real-Time PCR System, 3-year Extended Warranty

		StepOne™ Real-Time PCR System This 48-well low-throughput real-time PCR instrument makes it easy to get publication ready high quality data on day one. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR. StepOnePlus™ Real-Time PCR System with Laptop StepOnePlus™ Real-Time PCR System with Tower StepOnePlus™ Real-Time PCR System StepOnePlus™ Real-Time PCR System, 1-year Extended Warranty StepOnePlus™ Real-Time PCR System, 3-year Extended Warranty

		7500 Fast Real-Time PCR System The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. 7500 Fast Real-Time PCR System with Dell™ Notebook 7500 Fast Real-Time PCR System with Dell™Tower Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems 7500 Fast System Upgrade Kit 7500 Fast System SDS v1.4 21 CFR Part 11 Module High Resolution Melt (HRM) Software v2.0


		7900HT Fast Real-Time PCR System The 7900HT Fast Real-Time PCR System combines 384-well plate compatibility with fully automated robotic loading—and offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments. ABI PRISM® 7900HT Sequence Detection System User's Manual 7900HT Fast Real-Time PCR System with 384-Well Block Module 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module 7900HT System Automation Accessory Upgrade 7900HT System Standard 96-Well Block Upgrade Kit 7900HT System Standard 384-Well Block Upgrade Kit 7900HT System Fast 96-Well Block Upgrade Kit 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module 7900HT Fast Real-Time Service Upgrade 7900HT Fast Real-Time Service Upgrade with Computer

		ViiA™ 7 Real-Time PCR System The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab. Applied Biosystems® ViiA™ 7 Real-Time PCR System with 384-Well Block Applied Biosystems® ViiA™ 7 Real-Time PCR System with TaqMan® Array Applied Biosystems® ViiA™ 7 Real-Time PCR System with 96-Well Fast Block Applied Biosystems® ViiA™ 7 Real-Time PCR System with 96-Well Block ViiA™ 7 System Automation Accessory Robot (100–240 V) 384-Well Block Upgrade Kit TaqMan® Array Upgrade Kit 96-Well Fast Block Upgrade Kit 96-Well Block Upgrade Kit HRM Software Module for ViiA™ 7 System 21 CFR Part 11 Software Module for ViiA™ 7 System

The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.

TaqMan® Genotyper Software
TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

Literature

Product Bulletins

Posters

Mouse SNPbrowser™ Software: SNP Selection for Genetic Mapping and Genetic Monitoring in Laboratory Mice Strains

Application Notes

DNA Genotyping from Human FFPE Samples

For research use only. Not intended for any animal or human therapeutic or diagnostic use.