SNP Genotyping Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® block

Isolate and purify genomic DNA.

Products for Step 1: Prepare Sample

MagMAX™ DNA Multi-Sample Kit MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. MagMAX™ DNA Multi-Sample Kit MagMAX™-96 DNA Multi-Sample Kit MagMAX™-96 DNA Multi-Sample Kit (5 x 96 preps)

Select or design TaqMan® probes using the TaqMan® OpenArray® Plate Configurator or Custom TaqMan® Assay Design Tool respectively. QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with TaqMan® OpenArray® Master Mix in TaqMan® OpenArray® 384-well sample plate.

Load sample mixes onto QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plate using the QuantStudio™ 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. You can then perform on-line cycling on the QuantStudio 12K Flex system. To increase your throughput even more, as an option, you can amplify your on the Dual 96-well GeneAmp PCR System 9700 and perform an endpoint plate read using the QuantStudio 12K Flex system; TaqMan® SNP Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.

Recommended Products

		QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block High-throughput results with ease—the powerful QuantStudio™ 12K Flex system with OpenArray® block and AccuFill™ System combination enables the processing of thousands of samples per day without robotics. This product is not orderable online. For more information, please contact your local sales representative.
		Dual Flat Block GeneAmp® PCR System 9700 (Optional) GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The System consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with QuantStudio™ 12K Flex Real-Time PCR System with OpenArray block as an option to increase throughput. GeneAmp® PCR System 9700 and 9800 Fast Thermal Cycler, IQ User-Performed Test Procedures GeneAmp® PCR System 9700 and 9800 Fast Thermal Cycler, OQ/IPV User-Performed Test Procedures Dual Flat Block GeneAmp® PCR System 9700 Sample Module only OpenArray® Plate Adaptor for Dual Flat Block GeneAmp® PCR System 9700 OpenArray® Plate Compression Pad for Dual Flat Block GeneAmp® PCR System 9700 Dual Flat Block GeneAmp® PCR System 9700 GeneAmp® PCR System 9700 and 9800 Fast Thermal Cycler, IQ/OQ User-Performed Test Procedures GeneAmp® PCR System 9700,  Base Module Dual Flat Block GeneAmp® PCR System 9700, 3-year Extended Warranty Dual Flat Block GeneAmp® PCR System 9700, 1-year Extended Warranty

The data is plotted in an allelic discrimination plot with FAM fluorescence signal on the y-axis and VIC fluorescence signal on the x-axis. Software tools enable converting the raw data into genotype calls (e.g., homozygotes and heterozygotes.)

Software

		TaqMan® Genotyper Software TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.