TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block

The QuantStudio™ 12K Flex Real-Time PCR System helps streamline targeted discovery and validation, pathogen detection, and biomarker identification and screening, at a scale previously unattainable. Achieve the highest sample throughput for mid-density gene expression analysis - in a single day, one user can generate >42,000 gene expression data points without the use of robotics.

  • More replicates—each plate generates 2,600 data points, enabling you to include the technical replicates needed for better statistical power
  • Simple workflows—each QuantStudio™ 12K Flex OpenArray® Plate, encased in an alloy bottom for easy handling, comes preloaded with the assays of your choice- just add sample and master mix, cycle, and image
  • Experimental flexibility—specify the inventoried TaqMan® Gene Expression Assays you need, and select the OpenArray® Plate format that best meets your project needs
  • Reliable and economical—TaqMan® MGB probe chemistry in 33 nL reactions reduces reagent usage

TaqMan® OpenArray® Gene Expression Workflow on QuantStudio™ 12K Flex System:

Isolate and purify total RNA.

Recommended Products

 

MagMAX™ DNA Multi-Sample Kits
MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. MagMAX™ kits are available in both tube format (for manual processing) and in 96-well plate format (compatible with automated processing).

Publications & Literature

Literature

Publications

Select Language