What Can You Do With qPCR?
Gene Expression Analysis
Gene expression or messenger RNA (mRNA) analysis is the most commonly-used application for qPCR. Researchers reverse transcribe RNA, then use the cDNA produced as a template in qPCR reactions to detect and quantitate gene expression products.
- Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative
- One-Step vs. Two-Step Real-Time RT-PCR
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From miRNA profiling, to quantitation and detection of specific miRNAs, the TaqMan® MicroRNA Assay product line can help you quickly identify the miRNAs present in your sample and perform relative quantitation.
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Digital PCR is a new implementation of familiar qPCR technology that yields digital rather than analog data. It can provide absolute (rather than relative) quantitative target information with better sensitivity and precision than traditional qPCR, without reference to nucleic acid standards or controls.
Vogelstein B, Kinzler KW (1999) Digital PCR. Proc Natl Acad Sci USA, vol. 96 no. 169236–9241
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Genetic Variation Analysis
Real-time PCR instruments are commonly used for analysis of genomic variations. These techniques are not quantitative and for genotyping using allele-specific TaqMan® Assays, data are collected after thermal cycling is complete (not in real-time as the reaction progresses). Because these applications require a real-time PCR system, rather than a simple thermal cycler, in practice, they are typically considered real-time PCR applications.
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