Your instrument may not be adequately calibrated
Instrument calibration
To ensure optimal performance of your thermal cycler or sequence detection system, Applied Biosystems strongly recommends that you regularly calibrate the system. Calibration schedules vary by instrument; refer to the table below for the types of calibration and appropriate intervals.
Instrument calibration intervals
| Calibration Type | Instrument | Interval |
| ROI (Spatial) | 7300/7500/7500 Fast Real-time PCR Systems StepOne™ / StepOnePlus™ Systems | Every 6 months or As needed to verify the instrument’s performance |
| Background | 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems | Every month or Before performing a pure dye calibration or When installing an uncalibrated block |
| Pure dye spectra | 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems | Every 6 months or When installing an uncalibrated block |
| Instrument verification | 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems | Every 6 months or As needed to verify the instrument’s performance |
