Platinum®Taq DNA Polymerase
 | Platinum® Taq DNA Polymerase is a hot-start enzyme that has been trusted by researchers for robust, reliable amplification for years. This antibody-mediated, hot-start product provides high specificity for use in everyday PCR applications ranging from cloning to genotyping to mutagenesis. |
Platinum® Taq Technology
Platinum® Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures. Due to specific binding of the inhibitor, Platinum® Taq DNA Polymerase is provided in an inactive form. The DNA polymerase is then activated in a temperature-dependent manner (at 94ºC) during the start of PCR. Once dissociated from the inhibitor, the Taq DNA polymerase regains its full activity.
Benefits of Platinum® Taq DNA Polymerase
More Versatility for PCR products up to 10 kb
Use Platinum® Taq DNA Polymerase for expected PCR products up to 10 kb. The optional KB Extender gives more versatility to the Platinum® Taq DNA polymerase in PCR assays.
Panel A. A 4.1-kb region of genomic target was amplified from 200-400 ng K562 genomic DNA without KB Extender (Lanes 1-2), with KB Extender at 2% final concentration (Lanes 3-4) using Platinum® Taq DNA Polymerase; the same products amplified with Competitor DNA Polymerase (Lanes 6-7). Each reaction was performed in duplicate.
Panel B. A 10-kb region of genomic target was amplified from 200-400 ng K562 genomic DNA without KB Extender (Lanes 1-2), with KB Extender at 2% final concentration (Lanes 3-4) using Platinum® Taq DNA Polymerase; the same products amplified with Competitor DNA Polymerase (Lanes 6-7). Each reaction was performed in duplicate.
Broader Magnesium Range
A 2.8-kb region of the human β-globin gene was amplified from 100 ng of human genomic DNA. Using human genomic DNA, the amplification of a 2.8 kb region was evaluated at 1.0, 1.4, 1.8, 2.2, 2.6, and 3.0 mM magnesium (Lanes 1-6, respectively).
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Improved specificity with Platinum® Taq DNA Polymerase
 | One unit of Platinum® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 min at 74ºC.
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Panel A: Detection of cloned HIV DNA in human genomic DNA. 1,000 copies of plasmid DNA with the HIV gag region was mixed with 100 ng of genomic DNA and amplified with primers SK38 and SK39.
- Lane 1. Taq DNA Polymerase with room temperature assembly
- Lane 2. Taq DNA Polymerase with manual hot start by addition of enzyme at 94ºC
- Lane 3. Platinum® Taq DNA Polymerase with room temperature assembly
Panel B: Amplification of 4.1 kb of human ß-globin from 100 ng of human genomic DNA.
- Lane 1. Taq DNA Polymerase with room temperature assembly
- Lane 2. Taq DNA Polymerase with assembly on ice and placing in a preheated (80ºC) thermal cycler
- Lane 3. Platinum® Taq DNA Polymerase with room temperature assembly.temperature assembly
More Coverage for More Amplicons
Products amplified with Platinum® Taq DNA Polymerase (A); the same products amplified with Competitor DNA Polymerase (B). PCR reactions were run using 1 ng of template DNA and 1.25 units of enzyme in each 50 µL reaction. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Each reaction was performed in duplicate.
A. Platinum® Taq DNA Polymerase B. Competitor DNA Polymerase
High Specificity and Yield Comparison of Platinum® Taq DNA Polymerase to Competitor Polymerase
The data show a summary of two replicates for each of 40 amplicons, indicating the average specific yield for each amplicon and the standard deviation. Platinum® Taq PCR reactions were performed using 1 ng of template DNA per reaction and the manufacturer’s recommended cycling conditions. Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Data shown here compare Platinum® Taq DNA Polymerase (red) with Competitor Taq DNA Polymerase (green).
The data show a summary of two replicates for each of 40 amplicons, indicating the average specificity for each amplicon and the standard deviation. Platinum® Taq PCR reactions were performed using 1 ng of template DNA per reaction and the manufacturer’s recommended cycling conditions. Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Data shown here compare Platinum® Taq DNA Polymerase (red) with Competitor Taq DNA Polymerase (green).
