Fast PCR Enzymes
Fast PCR shortens cycling times from 2 hours or more to as little as 25 minutes through the use of special fast PCR enzymes combined with Fast, low volume thermal cycling. Applied Biosystems® offers you a choice of Fast PCR reagents, optimized to work with your application. When combined with the Applied Biosystems® Veriti® 96-well Fast Thermal Cycler, your thermal cycling becomes faster than ever before.
Which Fast PCR Enzyme is Right for You?
|Basic Choice for Fast PCR||Preferred Choice for Fast PCR||Ultimate Choice for Fast PCR|
|Time to Results||25 minutes (500bp)||35 minutes (500bp)||25 minutes (500bp)|
|Amplicon Size||up to 5 kb||up to 5 kb||up to 8 kb|
|Order Now||Order Now||Order Now|
Fast PCR Enzyme Selection Table
Use your application or template type to select the Fast enzyme that's right for you!
|Product||Time to Results||Broader Template Types||High Throughput||Sequencing Applications||Genotyping Applications||Cloning Applications||Mutagenesis Applications|
|GeneAmp® Fast PCR Master Mix|
|AmpliTaq Gold® Fast PCR Master Mix, UP|
PCR Results in 25 Minutes
This hot-start enzyme delivers fast PCR on sequences up to 2 kb in under 50 minutes and standard 500 bp targets from genomic DNA in under 25 minutes.
Sensitive and specific, you can detect a single copy of a gene in 10 ng of human genomic DNA. If you are speeding up your workflows for applications such as genotyping or endpoint PCR, GeneAmp® Fast PCR Master Mix is for you.
Fast PCR for Sequencing Applications
Brings PCR reaction times down to as little as 40 minutes and delivers specific, high-yield amplicons for easy, high quality sequencing data on par with standard PCR reagents. Designed for use with your existing primers.
Provides sensitive, reproducible results. Detect up to 10 copies of a single gene in 10 ng of genomic DNA.
Fast PCR Reaction Times
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|Figure 1: Decreased Thermal Cycling Times|
Reduce your cycling times down from over 2 hours to as little as 25 minutes with the GeneAmp® Fast PCR Master Mix.
The AmpliTaq Gold® Fast PCR Master Mix has been optimized to provide quick amplification for use in sequencing applications.