Digital PCR is a new approach to nucleic acid detection and quantification. It offers a different method for absolute quantification and rare allele detection relative to conventional real-time quantitative PCR.
Digital PCR works by partitioning a sample into many individual real-time PCR reactions; some portion of these reactions contain the target molecule (positive) while others do not (negative). Following PCR analysis, the fraction of negative reactions is used to generate an absolute count of the number of target molecules in the sample, without reference to standards or endogenous controls.
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Digital PCR for Absolute Quantitation
Digital PCR is quantitative PCR method that can be used for absolute target quantitation.
|Applications||Advantages of Digital PCR|
Digital PCR on QuantStudio® 12K Flex Real-Time PCR System
The QuantStudio® 12K Flex Real-Time PCR System enables digital PCR experiments at a scale previously unattainable - in a single day, one user can generate >49,000 digital PCR data points, without the use of robotics. Other features of the system include:
- Accuracy and sensitivity—detect and count individual molecules to quantify viral load, gDNA or cDNA targets, plasmids, or next-generation sequencing libraries.
- Speed—produce up to 192 digital answers in a 3 hour run.
- Flexibility—use your existing real-time PCR assays and test from one to 48 assays/sample dilutions per plate, with a combination of 4 plates.
- Wide dynamic range—with as few as 64 data points per replicate group, a dilution series can easily be loaded into the QuantStudio® Digital PCR Plate, expanding the range of sample concentrations which can be analyzed to produce a digital answer.
- Intuitive and economical to use—software includes a Poisson calculator, and measures values in real-time, to help you design your digital PCR experiment, eliminating optimization time and minimizing sample usage.
Digital PCR Workflow
Regulatory Status: For Research Use Only. Not for human or animal therapeutic or diagnostic use.