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Total RNA Purification

RiboMinus™ Technology

RiboMinus™ technology is designed to enrich the whole spectrum of RNA transcripts by selectively depleting ribosomal RNA molecules (rRNA), regardless of their polyadenylation status or the presence of of a 5'-cap structure. The RiboMinus™ method has been shown to remove  the vast majority of the most abundant ribosomal RNA molecules (up to 99.9%) to allow for greater interrogation of less abundant transcripts.

RiboMinus™ Workflow

  • RiboMinus™ products use a novel purification technology that enriches the RNA transcript spectrum by selective depletion of rRNA transcripts from total RNA.
 Step 1
The protocol begins with total RNA purified from culture, tissues, or blood using a method of choice (e.g., PureLink™ RNA Mini Kit, TRIzol® Reagent)

 Step 2
The total RNA is then hybridized with Locked Nucleic Acid (LNA) probes specific for abundant ribosomal RNA molecules.

 Step 3
Next, the unwanted rRNA are separated from the RiboMinus™- enriched complexes using RiboMinus™ Magnetic beads.

 Step 4
The RiboMinus™-enriched RNA sample is then concentrated using ethanol precipitation or a silica spin column step.


Step 5
The enriched RNA fraction is now ready for downstream processing (e.g., microarray, RNA-Seq).


  		• LNA™ (Locked Nucleic Acid)
    The structure of the LNA™ (Locked Nucleic Acid) monomer (see figure below) consists of a ribonucleoside linked between the 2′ oxygen and 4′ carbon atom of the methylene ring (Braasch & Corey, 2001). This configuration locks the sugar backbone resulting in an increase in Tm (melting temperature). Incorporation of 3 LNA™ monomers into an oligonucleotide does not affect the ability of the oligonucleotide to bind DNA or RNA but increases the stability of the oligonucleotide/RNA complex (McTigue et al., 2004). Oligonucleotides containing LNA™ are used in hybridization assays requiring high specificity and reproducibility