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E-Gel® CloneWell SYBR Safe™ gels & the E-Gel® iBase™ Power System

If you are extracting your DNA band for cloning, you need the E-Gel® CloneWell 0.8% SYBR Safe™ gels and the E-Gel® iBase™ Power System. When used together, you’ll be able to extract your DNA bands of interest from the gel without any additional purification steps, saving you time and effort while maximizing the amount of DNA you recover.

Smarter-by-design

  • The E-Gel® CloneWell gel features two rows of wells (Figure 1). To extract your DNA band of interest without gel purification, simply load your sample into the top row and electrophorese until your band moves into the second row of wells. Then use a pipette to easily remove your purified DNA band. Guides on the gel cassette help you track your band of interest so you know when your DNA of interest is ready for retrieval. There is no need to cut a gel slice and risk losing your DNA during a gel purification step. The 0.8% agarose concentration is ideal for separating bands up to 5 kb in length. SYBR Safe™ DNA Gel Stain is incorporated into the gel itself, allowing you to visually monitor migration on a Safe Imager™ Blue-Light Transilluminator.

A base designed for tracking

  • E-Gel® CloneWell 0.8% SYBR Safe™ gels run in the E-Gel® iBase™ Power System. This self-contained device includes a built-in power supply. Simply connect the AC adaptor provided and plug into an electrical outlet. View program selection and running time on the easy-to-read LCD display. Preset programs are available for various gel types or you can manually set your own run times. A reverse program allows you to change the field direction, ensuring complete capture of DNA. In addition, the E-Gel® iBase™ Power System includes an automatic shutoff feature, so you won’t overrun your gel. The E-Gel® iBase™ Power System and the E-Gel® Safe Imager™, a blue light transilluminator are designed to work together (Figure 2) so you can safely watch the DNA bands migrate in the gel.

Figure 1. The E-Gel® CloneWell 0.8% SYBR Safe™ gel. The two rows of wells in the CloneWell gels allow you to load samples in the top row and capture separated bands in the bottom row.

Figure 2. For maximum convenience and safety, run E-Gel® CloneWell™ gels in the E-Gel® iBase™ Power System and E-Gel® Safe Imager™ Real-Time Transilluminator.

Improved cloning efficiencies

  • Get significantly higher cloning efficiency with DNA extracted from CloneWell gels as compared to samples extracted from self cast gels. DNA recovered from CloneWell gels is compatible with all common cloning methods (Figure 3).


    Figure 3. Comparison of cloning results for DNA retrieved from E-Gel® CloneWell 0.8% SYBR Safe™ gels in three different cloning methods.

    In parallel experiments, the same 2Kb fragment was either recovered from CloneWell gels, or from a SYBR Safe™-stained gel slice using a commercially available gel extraction kit. The purified fragments were cloned into plasmids using restriction cloning, TOPO® cloning, or Gateway® cloning methods. For restriction cloning, the fragments were cloned into the pZErO-2 plasmid using T4 DNA Ligase. TOPO® cloning was performed using the TOPO® TA Cloning® Kit (with pCR®2.1-TOPO®), and for Gateway® cloning pDONR™ 221 was used.

Buy together and save

  • The E-Gel® CloneWell™ 0.8% SYBR Safe™, E-Gel® iBase™ & E-Gel® Safe Imager™ Starter Kit includes 18 E-Gel® CloneWell™ gels, an E-Gel® iBase™, an E-Gel® Safe Imager™, and an E-Gel® High Range DNA Ladder with enough material for 100 applications . Save money by purchasing the combo instead of buying these products separately.
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