D-LUX™ Performance vs. TaqMan® Assays
| The D-LUX™ Detection Platform provides a sensitive, cost-effective alternative to probe-based qPCR detection methods. With TaqMan® probes, you need a pair of PCR primers in addition to a dual-labeled probe that hybridizes to the internal portion of the amplicon. Using the D-LUX™ platform, all you need is one fluorogenic primer labeled with a single reporter dye, and one corresponding unlabeled primer. The fluorogenic primer can be either forward or reverse. The result is simple primer design with fast and inexpensive production, allowing you to analyze more genes at a lower cost compared to TaqMan® Probes with added conveniency of melting curve analysis capability. The D-LUX™ Detection Platform offers improved detection chemistry with specificity and sensitivity equal to TaqMan® probes (Figure 1), improved signal/background ratio, and an expanded array of software design features and functions for fast and easy primer design and higher performance primers. |
Figure 1 —D-LUX™ Detection Platform vs. TaqMan® Probes
Side-by-side comparison of qPCR with LUX™ Primers designed using D-LUX™ Designer(A) and TaqMan® probe detection system using ABI's Gene Expression Assay (B) for human Human NROB2 (NM_021969). Amplification was performed using Platinum® qPCR SuperMix-UDG using primer and probe amounts recommended by manufacturers. Human HMBS ORF clone was used with serial 10-fold dilution starting at 107 copies per reaction. Reactions were incubated for 2 min at 50°C, 2 min at 95°C, and 50 cycles of 15 sec 95°C, 30 sec at 55°C, and 30 sec at 72°C, followed by a melting curve using an ABI PRISM® 7700 instrument.
Side-by-side comparison of qPCR with LUX™ Primers designed using D-LUX™ Designer(A) and TaqMan® probe detection system using ABI's Gene Expression Assay (B) for human Human NROB2 (NM_021969). Amplification was performed using Platinum® qPCR SuperMix-UDG using primer and probe amounts recommended by manufacturers. Human HMBS ORF clone was used with serial 10-fold dilution starting at 107 copies per reaction. Reactions were incubated for 2 min at 50°C, 2 min at 95°C, and 50 cycles of 15 sec 95°C, 30 sec at 55°C, and 30 sec at 72°C, followed by a melting curve using an ABI PRISM® 7700 instrument.
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