qRT-PCR Direct from Cells - Cells-to-CT™ Kit
Ready to perform. Right out of the box.
Figure 1. Cells-to-CT™ Kits require 10 minutes or less to release nucleic acids into a cell lysate solution at room temperature that is compatible with the included reverse transcription and real-time PCR reagents.
- Extraordinary Value - complete, pre-optimized expression workflows in a single box are robust and reliable
- Extraordinary Ease - prepare samples at room temperature in 10 minutes or less, including DNase treatment
- Extraordinary Performance - results equivalent to purified RNA with validated accuracy, reproducibility, and sensitivity
Figure 1. Cells-to-CT™ Kits require 10 minutes or less to release nucleic acids into a cell lysate solution at room temperature that is compatible with the included reverse transcription and real-time PCR reagents.
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Cells-to-CT™ Kits take you from cultured cells to real-time PCR results with the fastest, easiest, and most robust workflows available today. A breakthrough cell lysis and RNA stabilization technology completely eliminates the need for RNA purification. Because samples can be processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility. However, Cells-to-CT™ Kits don’t stop at sample preparation. They integrate the lysis technology into complete, optimized expression workflows that include reverse transcription reagents and high performance TaqMan® or SYBR® Green-based Master Mixes.
All components of the Cells-to-CT™ Kits have been optimized for consistent and reliable performance, which removes the guesswork involved in assembling separate RNA purification, RT, and qRT-PCR kits. All Cells-to-CT™ Kits have been validated with hundreds of primer sets or TaqMan® Gene Expression Assays with diverse gene expression levels for added quality assurance.
To determine optimal performance for your particular experimental conditions, the TaqMan® and SYBR® Green Cells-to-CT™ Control Kits are available. Amplification of β-actin functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells. Amplification inhibition can be monitored using the included Xeno™ RNA Control. The kits include Xeno™ RNA Control, and Xeno™ RNA Control and β-actin TaqMan® Assays or primer sets.
All components of the Cells-to-CT™ Kits have been optimized for consistent and reliable performance, which removes the guesswork involved in assembling separate RNA purification, RT, and qRT-PCR kits. All Cells-to-CT™ Kits have been validated with hundreds of primer sets or TaqMan® Gene Expression Assays with diverse gene expression levels for added quality assurance.
To determine optimal performance for your particular experimental conditions, the TaqMan® and SYBR® Green Cells-to-CT™ Control Kits are available. Amplification of β-actin functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells. Amplification inhibition can be monitored using the included Xeno™ RNA Control. The kits include Xeno™ RNA Control, and Xeno™ RNA Control and β-actin TaqMan® Assays or primer sets.
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