Real-Time PCR
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Expertise in dye chemistries and oligonucleotide labeling has allowed Invitrogen to develop powerful qPCR detection systems. All qRT-PCR kits include SuperScript® III reverse transcriptase to provide consistent, reliable cDNA synthesis. The addition of uracil DNA glycosylase (UDG) prevents carryover contamination that can lead to false positives. All of Invitrogen's qPCR reagents are tested on a variety of qPCR cyclers, allowing us to support our products on virtually any instrument. To find the optimized Invitrogen qPCR or qRT-PCR reagent format that's right for you, find your desired format or starting material below. |
qPCR
If you are using cDNA or genomic DNA as your template, the EXPRESS qPCR reagent system is optimized to produce superior results on your fast cycling or standard instruments.
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Two-Step qRT-PCR
If you are using RNA as your template and want flexible qRT-PCR with the choice of primers for cDNA synthesis, and the ability to save cDNA for later use.
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One-Step qRT-PCR
If you are using RNA as your template and want convenient, high-throughput qRT-PCR amplification enzymes premixed with reverse transcriptase for fewer pipetting steps and reduced risk of contamination.
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qRT-PCR Direct from Cells
If you want highly sensitive and specific real-time qPCR directly from cells, without the need for an RNA purification step.
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