SuperScript® III Reverse Transcriptase

Superscript® III RT, a proprietary mutant of SuperScript® II RT, is the best-performing Reverse Transcriptase available for gene expression studies. SuperScript® III RT delivers more full length cDNA, superior cDNA yields, and is fully active at 50C, giving increased primer specificity

  • Reduced RNase H activity
  • A half-life of 220minutes at 50C, for the highest cDNA yields
  • Full activity at 50C for increased specificity with gene-specific primers

SuperScript® III Reverse Transcriptase Kit formats

Reverse Transcriptase Kit

SuperScript® VILO™ cDNA Synthesis kit

Variable input, linear output (VILO) to increase the dynamic range of your qRT-PCR assays
  • Increased cDNA yields with reduced qPCR inhibition
  • Accurate and sensitive quantitation across the broadest range of RNA input
  • (1 pg to 2.5ug of RNA per 20ul cDNA rxn)
  • Less qPCR inhibition for better sensitivity
  • Suitable for all qPCR detection methods such as SYBR® Green or fluorogenic primer/probe-based chemistry

 SuperScript® VILO™ cDNA Synthesis kit

RT-PCR kits

SuperScript® III RT provides improved performance in a variety of gene expression applications and is ideal for either one-step RT-PCR or two-step RT-PCR.
  • Reduced RNase H activity for more full length cDNA
  • A half-life of 220 minutes at 50°C, for the highest cDNA yields
  • Full activity at 50°C, for increased specificity with gene-specific primers
There are two approaches for RT-PCR: two-step RT-PCR and one-step RT-PCR. Two-step RT-PCR is useful for detecting multiple messages from a single RNA sample. One-step RT-PCR is easier to when processing large numbers of samples and helps minimize carry-over contamination because tubes are not opened between cDNA synthesis and amplification.

  • SuperScript® III RT-PCR kits
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