Reverse Transcription & cDNA Synthesis
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Choosing the correct enzyme for reverse transcription and cDNA synthesis is critical to obtaining high yields of quality full length cDNA with full gene representation. Selecting a reverse transcriptase enzyme with high temperature performance is also important when transcribing RNA with secondary structure or when working with gene-specific primers. Reverse transcription with reduced RNase H activity reverse transcriptase enzymes produces more full length cDNA and greater gene representation. The SuperScript® III Reverse Transcriptase is the most widely used brand of enzymes for cDNA synthesis, due to their proven ability to deliver reliable and consistently high yields of fully representative full length cDNA. Learn more about SuperScript® III Reverse Transcriptase Learn more about RT-PCR kits |
Reverse Transcription
| Reverse transcription enzymes and first-strand synthesis kits for reliable, quality cDNA. Learn more about Reverse Transcription |
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cDNA Synthesis Direct from Cells
| Highly sensitive and specific cDNA synthesis directly from cells, without the need for an RNA purification step. Learn more about qRT-PCR Direct from Cells |
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