RT-PCR
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There are two approaches for RT-PCR: two-step RT-PCR and one-step RT-PCR. Two-step RT-PCR is useful for detecting multiple messages from a single RNA sample. One-step RT-PCR is easier to when processing large numbers of samples and helps minimize carry-over contamination because tubes are not opened between cDNA synthesis and amplification. SuperScript® III RT provides improved performance in a variety of gene expression applications and is ideal for either one-step RT-PCR or two-step RT-PCR.
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One-Step RT-PCR
Convenient, high-throughput RT-PCR. Amplification enzymes premixed with reverse transcriptase for fewer pipetting steps and reduced risk of contamination.
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Two-Step RT-PCR
Flexible RT-PCR. Choice of primers for cDNA synthesis, and ability to save cDNA for later use.
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