| Calcium Indicator | Application |
| Fluo-4 Direct™ | Fluo-4 Direct™ Calcium Assay offers a homogenous, add-and-read Fluo-4 formulation ideal for HTS applications. The assay contains a background-suppressing quencher which allows for a large assay window even in the presence of serum-containing media. |
| Fluo-3 | Fluo-3 is the first generation of the Fluo family of products. Fluo-3 imaging has revealed the spatial dynamics of many elementary processes in Ca2+ signaling. Since about 1996, Fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. |
| Fluo-4 AM | Fluo-4 AM is the market-leading fluorescent calcium assay reagent that does not contain a background suppressing quencher dye. Fluo-4 AM, is an analog of fluo-3 with the two chlorine substituents replaced by fluorines. This fairly minor structural modification results in increased fluorescence excitation at 488 nm and consequently higher signal levels for confocal microscopy, flow cytometry, and microplate screening applications. |
| Fluo-4 NW | Fluo-4 NW provides the same advantages as Fluo-4 AM but is an improved formulation that includes PowerLoad™ and requires no washes after media removal. |
| Premo™ Cameleon | The combination of the Premo™ Cameleon Calcium Sensor and the efficient, ready-to-use BacMam technology in our first Premo™ product offers a unique, genetically encoded FRET-based biosensor which can detect real-time calcium flux in live cells over several days and cell divisions. |
| Fura-2 | Fura-2 has become the dye of choice for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, fura-2 exhibits an absorption shift of the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. |
| Indo-1 | Indo-1 is a preferred dye for flow cytometry, where it is more practical to use a single laser for excitation — usually the 351–364 nm spectral lines of the argon-ion laser — and monitor two emissions. The emission maximum of indo-1 shifts from ~475 nm in Ca2+-free medium to ~400 nm when the dye is saturated with Ca2+. |
