Tango™ GPCR Assay System
The Tango™ GPCR assay system provides a readout that is:
Life Technologies Tango™ GPCR Assay System (Figure 1) is a proprietary platform based upon ligand binding to a specific G protein-coupled receptor (GPCR) that triggers desensitization, a process mediated by the recruitment of protease-tagged arrestin proteins to the activated receptor. The protease-tagged arrestin cleaves a protease site fused to the C-terminus of the GPCR, releasing a non-native transcription factor.
This transcription factor immediately enters the nucleus, thus bypassing additional signaling intermediates. There, the transcription factor directly regulates transcription of a beta-lactamase reporter construct, which is measured upon addition of the live-cell substrate (Figure 2). The blue to green fluorescence ratio obtained directly correlates to receptor modulation by agonists or antagonists. Activation of the reporter gene provides a quantifiable measurement of the degree of interaction between the target receptor and the protease-tagged arrestin partner.
Validation with a diverse array of GPCRs
Life Technologies Tango™ GPCR assays have been validated for a diverse array of GPCRs, including receptors related to each of the major G protein pathways and activated by a variety of ligand types:
Agonist and Antagonist Assays
Tango™ technology is available on a custom basis for client-directed targets. Engineered cell lines are provided as a validated master cell line stock. Cells can also be provided on-demand in either 1-10 plate/vial configurations in either a dividing or division arrested format.
Screening and Profiling