Cell-Based GPCR Reporter Assays

Cell-Based GPCR Reporter Assays - G-Protein Coupled Receptors

The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. Invitrogen’s experienced team of GPCR biologists has generated a highly validated and novel family of GPCR cell line products to enable your discovery programs.

The portfolio contains two distinct assay platforms each utilizing FRET as the assay readout: Tango™ GPCR cell lines and GeneBLAzer® GPCR cell lines. Each GPCR cell line platform is available in two cell states: Division Arrested non-dividing cells and normal dividing cells. Click through our cell line product table below and capability links to the right to see how we can help accelerate your drug discovery efforts.

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Select a GPCR receptor family

		Assay Performance Data		Dividing Cells	Division Arrested Cells	Available in
Family	Receptor	Geneblazer® Technology	Tango™ Technology	Catalog no.	Catalog no.	SelectScreen™ Service	Confirmed Readout
Acetylcholine (Muscarinic)	M1			K1710			Ca²+(Fluo-4)
Acetylcholine (Muscarinic)	M1			K1735	K1365	X	Ca²+(Fluo-4), Phospho-ERK2
Acetylcholine (Muscarinic)	M1			K1861			Ca²+(Fluo-4), Phospho-ERK2
Acetylcholine (Muscarinic)	CHRM2 (M2)			K1564		X	Ca²+(Fluo-4)
Acetylcholine (Muscarinic)	M3			K1716		X	Ca²+(Fluo-4)
Acetylcholine (Muscarinic)	M4			K1741		X	Ca²+(Fluo-4)
Acetylcholine (Muscarinic)	M4			K1818		X	Phospho-ERK2
Acetylcholine (Muscarinic)	M5			K1728		X	Ca²+(Fluo-4)
Adenosine	ADORA1			K1445	K1601	X
Adenosine	ADORA2A			K1530	K1345		cAMP
Adenosine	ADORA2B			K1780
Adenosine	ADORA3			K1837
Adrenoreceptor	ADRA1A			K1470	K1577	X	Ca²+(Fluo-4)
Adrenoreceptor	ADRA1B			K1471		X	Ca²+(Fluo-4)
Adrenoreceptor	ADRA2A			K1743		X	Ca²+(Fluo-4)
Adrenoreceptor	ADRA2A			K1604	K1607	X
Adrenoreceptor	ADRB1			K1553		X	cAMP
Adrenoreceptor	ADRB2			K1472			cAMP, ERK2
Adrenoreceptor	ADRB3			K1745	K1473	X	cAMP
Angiotension	AGTR1			K1443		X	Ca²+(Fluo-4), Phospho-ERK2
Angiotension-Like	AGTRL1			K1754	K1762	X
Bradykinin	BDKRB1			K1734	K1363	X	Ca²+(Fluo-4), Phospho-ERK2
Bradykinin	BDKRB2			K1790		X
Calcitonin	CALCR			K1480		X	cAMP
Calcitonin	CALCRL: RAMP1			K1437		X	cAMP
Calcitonin	CALCRL: RAMP2			K1438			cAMP
Calcitonin	CALCRL: RAMP3			K1439		X	cAMP
Calcium Sensing	CASR			K1833		X	Ca²+(Fluo-4)
Cannabinoid	CNR1			K1513		X
Cannabinoid	CNR2			K1512	K1567	X
Chemokine	CXCR1			K1807		X
Chemokine	CXCR2			K1521		X	Ca²+(Fluo-4)
Chemokine	CXCR3			K1829		X	Ca²+(Fluo-4)
Chemokine	CXCR4			K1779		X	Ca²+(Fluo-4)
Chemokine	CXCR6			K1563		X
Chemokine	CXCR7			K1832		X
Chemokine	CCR1			K1787	K1793	X	Ca²+(Fluo-4)
Chemokine	CCR2			K1809		X
Chemokine	CCR3			K1815		X
Chemokine	CCR4			K1799		X	Ca²+(Fluo-4)
Chemokine	CCR5			K1788		X	Phospho-ERK2, Ca²+(Fluo-4)
Chemokine	CCR6			K1761	K1765	X
Chemokine	CCR7			K1531		X	Phospho-ERK2, Ca²+(Fluo-4)
Chemokine	CMKLR1			K1527	K1551	X
Cholecystokinin	CCKAR			K1548	K1623	X	Ca²+(Fluo-4), Phospho-ERK2
Cholecystokinin	CCKBR			K1742	K1379	X	Ca²+(Fluo-4)
Complement Component	C5AR1			K1746	K1554	X	Ca²+(Fluo-4)
Corticotropin Releasing Factor	CRHR1			K1736	K1367	X	cAMP
Corticotropin Releasing Factor	CRHR2			K1733		X	cAMP

		Assay Performance Data		Dividing Cells	Division Arrested Cells	Available in
Family	Receptor	Geneblazer® Technology	Tango™ Technology	Catalog no.	Catalog no.	SelectScreen™ Service	Confirmed Readout
Dopamine	D1			K1485			cAMP
Dopamine	D2			K1708		X	Ca²+(Fluo-4), Phospho-ERK2
Dopamine	D5			K1784			Ca²+(Fluo-4)
Endothelial Differentiation Gene	EDG1			K1520		X
Endothelial Differentiation Gene	EDG2			K1519		X
Endothelial Differentiation Gene	EDG3			K1713	K1319	X	Ca²+(Fluo-4)
Endothelial Differentiation Gene	EDG3			K1848			Ca²+(Fluo-4)
Endothelial Differentiation Gene	EDG4			K1442		X
Endothelial Differentiation Gene	EDG6			K1622	K1757	X
Endothelial Differentiation Gene	EDG7			K1511	K1315	X	Ca²+(Fluo-4)
Endothelial Differentiation Gene	EDG7			K1849		X	Ca²+(Fluo-4)
Endothelial Differentiation Gene	EDG8			K1518		X
Endothelin	EDNRA			K1726	K1347	X	Ca²+(Fluo-4)
Endothelin	EDNRA			K1444		X
Endothelin	EDNRB			K1727	K1349	X	Ca²+(Fluo-4)
Formyl Peptide-Like	FPRL-1			K1532	K1357	X	Ca²+(Fluo-4)
Galanin	GALR1			K1616		X
Galanin	GALR2			K1811		X
Gastrin Releasing Peptide	GRPR			K1561	K1637	X
Ghrelin	GHSR			K1819		X	Ca²+(Fluo-4)
Gastric Inhibitory Polypeptide	GIPR			K1864		X
Glucagon	GCGR			K1855		X	cAMP
Glucagon	GLP1R			K1783		X	cAMP
Glucagon	GLP2R			K1543		X	cAMP
Gonadotropin Releasing Hormone	GnRHR			K1854		X	Ca²+(Fluo-4)
Histamine	H1			K1703	K1299	X	Ca²+(Fluo-4)
Histamine	H2			K1707	K1307	X	cAMP
Histamine	H3			K1466	K1509	X
Histamine	H4			K1465		X	Ca²+(Fluo-4)
KiSS1	GPR54			K1720	K1333	X	Ca²+(Fluo-4)
Leukotriene	LTB4R			K1748		X	Ca²+(Fluo-4)
Melanin-concentrating Hormone	MCHR1 (GPR24)			K1730		X	Ca²+(Fluo-4)
Melanin-concentrating Hormone	MCHR2 (GPR145)			K1852		X	Ca²+(Fluo-4)
Melanin-concentrating Hormone	MCHR2 (GPR145)			K1836		X	Ca²+(Fluo-4), Phospho-ERK2
Melanocortin	MC1R			K1724	K1341	X	cAMP
Melanocortin	MC2R			K1483	K1597	X
Melanocortin	MC3R			K1744	K1423	X	cAMP
Melanocortin	MC5R			K1740		X	cAMP
Melatonin	MTNR1A			K1669		X
Melatonin	MTNR1B			K1753		X
Motilin	MLNR			K1862
Neuropeptide B/W	GPR8			K1478		X
Neuropeptide S	NPSR1a			K1447	K1493	X	Ca²+(Fluo-4)
Neuropeptide S	NPSR1b			K1481		X	Ca²+(Fluo-4)
Neuromedin U	NMUR1			K1719		X
Neurotensin	NTSR1			K1781	K1773	X	Ca²+(Fluo-4)
Neuropeptide Y	NPY1R			K1803		X
Neuropeptide Y	NPY2R			K1479	K1501	X
Neuropeptide Y	NPY5R			K1782

		Assay Performance Data		Dividing Cells	Division Arrested Cells	Available in
Family	Receptor	Geneblazer® Technology	Tango™ Technology	Catalog no.	Catalog no.	SelectScreen™ Service	Confirmed Readout
Nicotinic Acid	GPR109A			K1791		X
Opioid	OPRD1			K1778		X
Opioid	OPRM1			K1523	K1599	X
Opioid	OPRK1			K1533		X	Ca²+(Fluo-4). Phospho-ERK2
Opioid	OPRK1			K1576		X	Phospho-ERK2
Opioid	OPRL1			K1714		X	Phospho-ERK2
Opioid	OPRL1			K1786		X	Ca²+(Fluo-4), Phospho-ERK2
Orexin	HCRTR1			K1446		X	Ca²+(Fluo-4)
Orexin	HCRTR2			K1737	K1369	X	Ca²+(Fluo-4)
Platelet Activating Factor	PTAFR			K1789		X	Ca²+(Fluo-4)
Prokineticin	PROKR2			K1856
Prolactin Releasing Peptide	GPR10			K1732		X	Ca²+(Fluo-4)
Prostanoid	PTGDR			K1739	K1373	X	cAMP
Prostanoid	CRTH2 (GPR44)			K1859		X
Prostanoid	PTGER1			K1851		X
Prostanoid	PTGER2			K1723		X	cAMP
Prostanoid	PTGIR			K1850		X
Prostanoid	TBXA2R			K1549		X	Ca²+(Fluo-4), Phospho-ERK2
Protease Activated	F2RL1			K1830		X
P2Y (Purinergic/ Pyrimidinergic)	P2RY2			K1722	K1337	X	Ca²+(Fluo-4)
P2Y (Purinergic)	P2RY4			K1863			Ca²+(Fluo-4)
P2Y (Purinergic)	P2RY6			K1717	K1327	X
P2Y (Purinergic)	P2RY11			K1729		X	Ca²+(Fluo-4)
P2Y (Purinergic)	P2RY14			K1858
Relaxin	RLN3R1			K1569		X
Secretin	SCTR			K1805		X
Serotonin	HTR1A (5HT1A)			K1857		X	Phospho-ERK2
Serotonin	HTR2B			K1847		X
Serotonin	HTR1D			K1524
Serotonin	HTR2A			K1562	K1613	X	Ca²+(Fluo-4)
Serotonin	HTR2C			K1877			Ca²+(Fluo-4)
Serotonin	HTR2C						Ca²+(Fluo-4)
Serotonin	HTR7			K1704		X
Somatostatin	SSTR1			K1570		X
Somatostatin	SSTR2			K1582		X
Somatostatin	SSTR5			K1575	K1573	X
Tachykinin	TACR1			K1801		X
Tachykinin	TACR2			K1844		X	Ca²+(Fluo-4), Phospho-ERK2
Tachykinin	TACR3			K1603		X
Vasopressin & Oxytocin	AVPR1a			K1715	K1323	X	Ca²+(Fluo-4), Phospho-ERK2
Vasopressin & Oxytocin	AVPR2			K1718	K1329	X	cAMP
Vasopressin & Oxytocin	OXTR			K1835		X
VIP & PACAP	VPAC1			K1705		X	cAMP
VIP & PACAP	VPAC2			K1706		X	cAMP
VIP & PACAP	ADCYAP1R1 (PAC1)			K1525	K1619	X	cAMP
Orphan	GPR1			K1839			cAMP
Orphan	GPR21			K1840
Orphan	GPR23			K1841
Orphan	GPR35			K1872		X
Orphan	GPR39			K1842
Orphan	GPR119			K1486		X
Orphan	GPR119			K1777	K1770
Orphan	GPR120 (short)			K1845
Orphan	GPR132 (G2A)			K1749