BacMam 2.0 GFP Transduction Control
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Easily Evaluate BacMam 2.0 in Your Cell Model
The BacMam GFP Transduction Control lets you experience the power and convenience of enhanced BacMam 2.0 technology, which greatly expands the efficiency and utility of this popular gene delivery platform. Cell types previously not compatible (neuronal cells) or only poorly transduced (some stem cells, CHO cells) with the technology are now transduced quantitatively in a simple, one-step process (Figure 1).
Easily evaluate BacMam 2.0 in your cell model before advancing to the more specialized BacMam reagents with the convenient BacMam 2.0 GFP Transduction Control. This control is based on our extremely bright and photostable emGFP, which lights up the entire cell in this non-targeted form.
) | Figure 1—Comparison of the transduction effieciency of BacMam 1.0 (top row) versus BacMam 2.0 (bottom row). T84 (adenocarcinoma) cells (left column) and ADSC (adipocyte-dervied stem cells) cells (right column) were transduced with the same conditions and particles per cell. BacMam 2.0 yields much more robust GFP expression under identical conditions. |
BacMam 2.0 GFP Movies
 Glial cells were co-transduced with BacMam GFP transduction control and CellLight™ Mitochondria-RFP and live cell imaging performed using a DeltaVision® Core microscope and standard FITC/TRITC filter sets. |  Mitochondrial transport along microtubules in rat hippocampal neonatal neurons transduced with BacMam GFP transduction control and CellLight™ Mitochondria-RFP and live cell imaging performed using a DeltaVision® Core microscope and standard FITC/TRITC filter sets. |  Growth cone dynamics in rat hippocampal neonatal neurons transduced with BacMam GFP transduction control and live cell imaging performed using a DeltaVision® Core microscope and standard FITC filter sets. |
Simple and Efficient
Using BacMam 2.0 reagents is as simple as it is efficient (Figure 2). Just mix BacMam 2.0 GFP with your cells, incubate overnight, and analyze GFP expression levels the next day or freeze for future use.
) | Figure 2—BacMam 2.0 workflow. Simply culture cells, add virus, incubate and visualize BacMam 2.0 fluorescent protein expression! |
Improved Performance of BacMam 2.0
BacMam technology is based on the use of an insect virus (baculovirus) for efficient gene delivery and expression in mammalian cells. The improved performance of BacMam 2.0 reagents is due to inclusion of elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry and genetic elements (enhanced CMV promoter and Woodchuck Posttranscriptional Regulatory Element) that boost expression levels (Figure 3). See Table 1 for a comparison of the transduction efficiencies of BacMam 1.0 and BacMam 2.0 across different cell types.
) | Figure 3—Comparison of BacMam 1.0 versus BacMam 2.0 viral particles. |
Choose the BacMam Reagent for Your Application
Easier to perform experiments in Biologically relevant cell models
Due to the high transduction efficiency and low cytotoxicity of BacMam 2.0, it’s easy to perform experiments in biologically relevant cell models (e.g., stem cells, neurons, and primary cells). For multicomponent systems that require stoichiometric expression, the transient expression and ability to control expression by simply varying the dose enables analysis and even screening of targets that have proven intractable using stable cell lines.
BacMam-based Reagents
To facilitate the study of live cells, we have generated a range of BacMam-based reagents including drug targets, biosensors, pathway analysis kits, and cellular imaging reagents.
Table 1. Comparison of BacMam 1.0 and BacMam 2.0 across different cell types
| BacMam 1.0 No enhancer | BacMam 1.0 With enhancer | BacMam 2.0 No enhancer | BacMam 2.0 With enhancer | |
|---|---|---|---|---|
| Cell lines | +/- | ++ | ++ | ++ |
| Primary cells | + | ++ | ++ | ++ |
| Stem cells | - | ++ | ++ | ++ |
| Neurons | - | - | ++ | ++ |
| Immortalized T-cells | - | - | +/- | + |
| Primary T-cells and B-cells | - | - | - | - |
- = transduction efficiency <10% | ||||
