LanthaScreen® Cellular Assays Validation Table

LanthaScreen® cellular assays are developed to ensure proper pharmacology toward the target of interest.  Each assay provides excellent response profiles, yields a Z´-factor above 0.6, and demonstrates the appropriate EC50/IC50 responses to known agonists or antagonists.  In addition, the cell lines used in LanthaScreen™ cellular assays are mycoplasma-negative and tested for optimal performance under a variety of experimental conditions.  Our goal is to provide effective tools with robust results to keep your screening program on track.

See the list of available LanthaScreen® cellular assays below, along with validation data and assay protocols specific to detection of each post-translational modification.

Target Substrate Modification Detected Cell Line Name Cell Line Cat.# Antibody Validation Packet Customer Protocol
AKT Phosphorylation of Ser473 LanthaScreen® AKT HEK293E K1615 LanthaScreen® Tb-anti-AKT [pSer473] Antibody    
ATF2 (19-106) Phosphorylation of Thr71 LanthaScreen® ATF2 (19-106) A549 K1679 LanthaScreen® Tb-anti-ATF2 [pThr71] Antibody
c-Jun (1-79) Phosphorylation of Ser73 LanthaScreen® c-Jun (1-79) HeLa K1680 LanthaScreen® Tb-anti-c-Jun [pSer73] Antibody
ERK2 Phosphorylation of Thr185/Tyr187 LanthaScreen® ERK2 U2OS K1587 LanthaScreen® Tb-anti-ERK2 [pThr185/pTyr187] Antibody
ERK2 Phosphorylation of Thr185/Tyr187 LanthaScreen® ERK2 A375 (B-RAF V600E) K1798 LanthaScreen® Tb-anti-ERK2 [pThr185/pTyr187] Antibody
IGF-1R Phosphorylation of Tyr Residues LanthaScreen® IGF-1R GripTite® K1834 LanthaScreen® Tb-PY20 Antibody
IkB Phosphorylation of Ser32 LanthaScreen® IkB GripTite® K1681 LanthaScreen® Tb-anti-pIkB [pSer32] Antibody
PDCD4 Phosphorylation of Ser457 LanthaScreen® PDCD4 HEK293E K1593 LanthaScreen® Tb-anti-PDCD4 [pSer457] Antibody
PRAS40 Phosphorylation of Thr246 LanthaScreen® PRAS40 HEK293E K1528 LanthaScreen® Tb-anti-PRAS40 [pThr246] Antibody
PRAS40 Phosphorylation of Ser183 LanthaScreen® PRAS40 HEK293E K1528 LanthaScreen® Tb-anti-PRAS40 [pSer183] Antibody
STAT1 Phosphorylation of Tyr701 LanthaScreen® STAT1 U2OS K1469 LanthaScreen® Tb-STAT1 [pTyr701] Antibody
STAT3 Phosphorylation of Tyr705 LanthaScreen® STAT3 GripTite K1488 LanthaScreen® Tb-STAT3 [pTyr705] Antibody
STAT5 Phosphorylation of Tyr694/Tyr699 LanthaScreen® STAT5 TF-1 K1598 Anti-STAT5 A/B [pTyr694/699] Antibody
STAT5 Phosphorylation of Tyr694/Tyr699 LanthaScreen® STAT5 U2OS (JAK2 V617F) K1588 Anti-STAT5 A/B [pTyr694/699] Antibody
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