CellSensor® Cell Lines
Effectively analyze signal transduction pathways of biological relevance.
Our CellSensor® Cell Lines can be used to:
Many intracellular signaling pathways converge at the transcriptional level, where transcription factors bind to DNA enhancer elements that regulate downstream transcription. We have used lentiviral vectors to produce a series of stable cell lines containing various response elements upstream of the beta-lactamase reporter gene.
When pathways leading to specific enhancer elements are activated in these cell lines, beta-lactamase is produced, which can then be detected in a homogeneous assay with our cell-permeable, ratiometric fluorescent LiveBLAzer™-FRET B/G substrate (Figure 1).
Learn more about GeneBLAzer® substrates.
For instrumentation set-up help or a list of validated instruments, please email us at email@example.com.
Life Technologies Posters
- Advancing drug discovery using a multi-pathway cell-based assay approach to compound selectivity profiling
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