Cellular Pathway Analysis Assays
 | Tools that help analyze compound efficacy in a pathway-specific physiological context are essential for drug discovery research. To address this need, Life Technologies offers three technology platforms for endpoint or proximity-oriented pathway analysis for use in high-throughput screening and profiling. To compile the components needed to make the right cellular assay for your research, use our new Drug Discovery Assay Maker™ web tool. |
Choose the right cellular pathway analysis assay for your research
| Analysis of an entire signal transduction pathway with a single end-point readout | Detection of post-translational modifications of specific protein substrates within a native cellular environment | Detection of post-translational modifications of specific protein substrates in the cell background of your choice | |||
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| Stable vs. Transient | Stable | Stable | Transient | ||
| Cellular engineering | Pathway-specific response element upstream of BLA reporter | GFP-substrate fusion, readout of endogenous kinases | GFP-substrate fusion, readout of endogenous kinases | ||
| Assay optimization required? | No, already optimized | No, already optimized | Yes, if using cell backgrounds other than recommended | ||
| Detection technology | FRET (coumarin to fluorescein) | TR-FRET (terbium-labeled antibody to GFP-substrate fusion) | TR-FRET (terbium-labeled antibody to GFP-substrate fusion) | ||
| Antibody-based assay? |
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| Total assay volume (384-well) | 40 µL | 40 µL | 40 µL | ||
| Overcoming interference | Background suppression dye in the substrate mixture | Time-resolved readout allows read after interfering signals have decayed | Time-resolved readout allows read after interfering signals have decayed | ||
| Plate reader method | Bottom-read | Top-read | Top-read | ||
| Cost per well | $ | $$ - $$$ | $$ - $$$ | ||
| Use in SelectScreen® Cellular Profiling Services | |
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| Request Info | Request Info | Request Info |
Elucidate your target pathways with multiple assay formats
Life Technologies offers three assay technologies for your cellular pathway analysis research.
- BacMam-enabled Cellular Assays are an efficient, easy-to-optimize, and robust method for interrogating specific signal transduction events in a cell background of choice.
- LanthaScreen™ cellular assays allow for detection of post-translational modifications of specific protein substrates within the native cellular environment.
- CellSensor® pathway reporter gene assays measure the transcriptional activation of a pathway-specific cellular response element.
