Cellular Pathway Analysis Assays

In drug discovery, there is a need for tools to analyze compound efficacy in a pathway-specific physiological context. To address this need, Invitrogen offers two technology platforms for endpoint or proximity-oriented pathway analysis for use in high-throughput screening and profiling.

Elucidate your target pathways with multiple assay formats

Portable cellular assay for post-translational modifications

BacMam-enabled Cellular Assays are an efficient, easy-to-optimize, and robust method for interrogating specific signal transduction events in a cell background of choice.
 
  • Portable assay compatible with most cell types, including primary and stem cells
  • Quickly develop assays in less than one week without having to generate stable cell lines
  • Simple, homogenous assay format utilizing a single antibody
  • Measure multiple modifications, such as phosphorylation, acetylation, or ubiquitination

Learn more about BacMam-enabled Cellular Assays


Post-Translational Modification Assays

LanthaScreen™ cellular assays allow for detection of post-translational modifications of specific protein substrates within the native cellular environment.

  • Analyze your compound’s effect at a specific step in a signaling pathway
  • Utilize a robust TR-FRET readout in a user-friendly HTS format
  • Interrogate specific key signaling events within a pathway

Learn more about the LanthaScreen™ cellular assay technology


Transcriptional Reporter Gene Assays

CellSensor® cell lines measure the transcriptional activation of a pathway-specific cellular response element.

  • Profile the effect of your compounds on disease relevant signaling pathways
  • Utilize the robust and ratiometric beta-lactamase reporter gene readout for HTS
  • Generate cellular pathway selectivity profiles of your compounds

Learn more about the CellSensor® technology