Omnia® Kinase Assays

Measure Kinase Activity in Real Time

  • Easy protocol—just mix, then read fluorescence in real time
  • Flexible—use recombinant kinases or crude cell lysates at low (μM) or high (mM) ATP concentration
  • Fast—get results in under an hour
  • Minimal interference—kinetic format corrects for false positives or negatives
Omnia® Kinase Assays utilize a fluorescence peptide substrate-based technology for the rapid, homogenous and sensitive detection of enzymatic activity. Results are measured under optimal kinetics, physiological (mM) ATP, and in real time without use of radioactive tracers or specialized equipment. Increases in kinase activity result in a corresponding increase in fluorescence. The kinetic format of the assay also corrects for false positives (compound auto fluorescence) and false negatives (quenching), minimizing interference. The assay can be used to select for both ATP-competitive and ATP non-competitive (allosteric) inhibitors. Omnia® Assays can be used with unmodified crude cell lysates, recombinant kinases or immunoprecipitated proteins.
 
Learn more about the Omnia® Kinase Principle.


For information on how to use Omnia® for analysis of cellular lysates or immunoprecipitated proteins, please contact us at discoverysciences@invitrogen.com.
Kinase (112 total) Best Kit Alternatives
AKT1ST1ST4, ST5, ST6
AKT2ST1ST4, ST5, ST6
AKT3ST1ST4, ST5, ST6
AMPKST23ST11
ARK5 ST31  
AURORA AST16ST5
AURORA BST16 
AURORA CST16 
BRSK1    
CAMK1D (CAMKIδ) ST23 
CAMK2A (CAMKIIα) ST23 
CAMK2D (CAMKIIδ) ST23 
CDC42BPA (MRCK/DMPK)ST18 
CDC42BPB (MRCK/DMPK)ST18 
CDK1/CyclinBST7 
CDK1/CyclinEST7 
CDK2/CyclinAST7 
CDK2/CyclinEST7 
CDK4/CyclinD1    
CDK5/p25NCKST7 
CDK5/p35ST7 
CDK6/CyclinD1    
CHEK1 (CHK1)ST11ST1, ST3
CHEK2 (CHK2)ST3ST11
CLK2ST15 
CLK3ST15 
CSNK1A1 (CK1)ST24 
CSNK1D (CKIδ)ST24 
CSNK1E (CK1ε)ST24 
CSNK1G1 (CK1γ1)ST24 
CSNK1G2 (CK1γ2)ST24ST21
CSNK1G3 (CK1γ3)ST24ST21
CSNK2A1 (CK2α1)ST22ST21
DAPK3ST1 
DCAMKL2    
DYRK1A    
GSK3αST13 
GSK3β (C-terminal His)ST13 
IKKβ (IKK2) ST20ST19
IKKε ST24 ST12, ST16
MAP3K10 (MLK2)ST6ST1, ST5
MAP3K11 (MLK3)ST8 
MAP3K9 (MLK1)ST8  
MAP4K2 (GCK) ST25ST26
MAP4K4 (HGK) ST32 ST26
MAP4K5 (KHS1)ST26ST25
MAPK1 (ERK2) ST17ST9
MAPK10 (JNK3)ST10 
MAPK11 (p38β)ST9 
MAPK12 (p38γ)ST7ST9, ST10
MAPK13 (p38δ)
ST10
MAPK14 (p38α)
 
MAPK3 (ERK1) ST17 
MAPK8 (JNK1) ST10 
MAPKAP-K2 (MK2) ST3 
MAPKAP-K3 ST3ST11
MAPKAP-K5 (PRAK) ST3 
MARK2 ST31 
MINK1ST8 
MLK1ST8 
MLK3ST8 
NEK1    
NEK2    
NEK4    
NEK6 ST27  
NEK9    
PAK1ST5ST1, ST6
PAK2ST5 
PAK3ST5 
PAK4ST5 
PAK6 ST5 
PAK7ST5 
PASKST6ST1, ST4, ST15
PHKG1ST3 
PHKG2ST3ST11, ST15
PIM1ST26  
PIM2ST14ST15
PKAST5ST1, ST6
PKCαST8 
PKCβIST8 
PKCβIIST8 
PKCδST8 
PKCεST8 
PKCηST8ST15
PKCγST8 
PKCιST8 
PKCθST8 
PKCζST8 
PKCμST8 ST3, ST11
PKN1 (PRK1)ST18 
PRKCN (PKD3)ST8ST3
PRKD2 ST8ST3, ST11
PRKG1ST5
PRKG2ST5ST1, ST4
PRKXST5ST1, ST6
ROCK1ST5ST14
ROCK2ST5ST6
RPS6KA1 (RSK1)ST6ST1, ST5
RPS6KA2 (RSK3)ST6ST1, ST5
RPS6KA3 (RSK2) ST6ST1, ST5
RPS6KA5 (MSK1) ST1 ST4, ST5, ST6
RPS6KA6 (RSK4)ST6ST1, ST5
RPS6KB1 (p70S6K)ST6 ST18
SGK (SGK1)ST1ST4, ST5, ST6
SGK2ST4ST1, ST5, ST6
SGKL (SGK3)ST4ST1, ST5, ST6
STK22B (TSSK2) ST31 
STK22D (TSSK1)
 
STK3 (MST2)
 
STK4 (MST1)
 
TAOK2 (TAO1)
 
TBK1 (IKK-ε)ST12 

Cascade Assays
Kinase (19 total)		 Direct Substrate
b-RAF  ST3
b-RAF, Catalytic   ST3
c-RAF (RAF1)   ST3
MAP2K1 (MEK1)   ST3
MAP2K2 (MEK2)   ST3
MAP2K6 (MKK6)   ST3
MAP3K2 (MEKK2)   ST3
MAP3K3 (MEKK3)   ST3
MAP3k8 (COT)   ST3
MAPK1 (ERK2) ST17 ST3
MAPK11 (p38β) ST9 ST3
MAPK12 (p38γ) ST7 ST3
MAPK13 (p38δ)
ST3
MAPK14 (p38α)
ST3
MAPK3 (ERK1) ST17 ST3
MAPK8 (JNK1) ST10 ST3
MAPK9 (JNK2)   ST3
MAPK10 (JNK3) ST10 ST3
PDK1   ST1
Kinase (77 total)Best Kit Alternatives
ABL1 Y6Y1, Y5, Y7
ABL1 E255KY6 
ABL1 G250EY6 
ABL1 T315IY6 
ABL1 Y253FY6 
ABLl2 (ARG)Y6 Y5
ALKY4 
AXL Y11  
BLKY5 Y6, Y14
BMXY5 
BTKY5Y6
CSF-1R (FMS)Y9 Y12
EGFR (ErbB1) Y12Y7
EGFR T790M Y12  
EGFR L858R Y10 Y12
EGFR L861Q Y12 Y7, Y9
EGFR1Y7Y8
EPHA1Y6Y5
EPHA2Y5Y3
EPHA4 Y12Y3
EPHA5   
EPHA8 Y12 Y5
EPHB1Y6Y5
EPHB2Y6 Y14
EPHB3Y6Y5
EPHB4Y5Y6
FERY5Y4
FGFR1 Y12Y7, Y8
FGFR2Y11 
FGFR3Y11 
FGFR3 K650E Y12Y8, Y9
FGFR4Y10Y11
FGRY5 Y1, Y12
FLT1 (VEGFR1)Y8Y7
FLT3 CDY5 Y4, Y14
FLT3 D835YY5Y9
FLT4 (VEGFR3) Y8Y7, Y12
FRKY5Y9, Y12
FYNY5Y2
HCKY5 Y2
IGF-1RY4Y11, Y12
IR (Insulin Receptor)Y4Y11, Y12
IRR (Insulin-related receptor)Y4 Y12
ITKY6 Y14
JAK2Y11Y7, Y10
JAK2 JH1 JH2 Y12  
JAK2 JH1 JH2 V617F Y12 Y7
JAK3Y7 Y12
KDR (VEGFR2)Y8 Y7, Y12
KITY9Y10, Y12
KIT T670I Y12Y9, Y10
LCKY5 Y6, Y14
LYN AY5Y1
LYN BY5Y1
MERTK (cMER)Y6Y9, Y12, Y14
MET (HGFR) Y12 Y14
MET (HGFR) M1250T Y9 Y12, Y14
MST1RY8 
MUSKY1Y4
NTRK1 (TRKA)Y8Y11, Y4
NTRK2 (TRKB)Y5 Y6, Y12
PDGFRA (PDGFRα)Y9Y1, Y12
PDGFRA D824VY9 Y12, Y14
PDGFRB (PDGFRβ) Y14Y6
PTK2 (FAK)Y3Y2
RET Y12Y7
RET V804LY8  
RET Y791FY11Y7
ROS1Y4Y12, Y11
SRCY2 
SRCN2   
SRMS Y12 Y6, Y14
SYKY7Y12
TEK (tie2)Y8Y5
TYRO3 (RSE)Y5 
YESY5Y2, Y8
ZAP70Y8 

Table 1. Ordering information for reagents

Reagents Cat. No.
Omnia® Ser/Thr Reaction BufferKB001A
Omnia® Tyr reaction BufferKB002A
Omnia® ATP SolutionAS001A

Omnia® Custom and Bulk Substrate Services

Should you not find your substrate of interest, Invitrogen also offers custom services for the generation of Omnia® peptide substrates. Omnia® substrates can be successfully produced for a wide variety of kinases. We also can provide milligram quantites of lyophilized substrate for your large screening projects.

Please contact us at discoverysciences@invitrogen.com for more information about these services or to request a quotation.
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