LanthaScreen® TR-FRET Tools for Assay Development
Flexible tools for TR-FRET assay development:
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If you don't see your antibody of interest on our list, let us label your antibody for you. Inquire about our custom labeling services.
LanthaScreen® anti-Epitope Tag Antibodies
Epitope tags facilitate the purification of proteins for use in many applications. Such tags can also be used to indirectly label proteins through the use of LanthaScreen® anti-epitope tag antibodies.
Indirect labeling of a protein through the use of an anti-epitope tag antibody can be advantageous when working with partially purified protein, or in cases in which the direct labeling of the protein would negatively impact protein activity.
Our products are available as kits or stand alone antibodies. Trial kits contain 100mL of TR-FRET dilution buffer (PV3574), which is recommended for antibody dilution, and a positive control peptide for the antibody when available. Europium antibodies are compatible with fluorophores purchased from Invitrogen or other suppliers including but not limited to Alexa Fluor® 647, Cy3, Cy5, and ULight dyes. Terbium antibodies are compatible with fluorescein or GFP.
Indirect labeling of a protein through the use of an anti-epitope tag antibody can be advantageous when working with partially purified protein, or in cases in which the direct labeling of the protein would negatively impact protein activity.
Our products are available as kits or stand alone antibodies. Trial kits contain 100mL of TR-FRET dilution buffer (PV3574), which is recommended for antibody dilution, and a positive control peptide for the antibody when available. Europium antibodies are compatible with fluorophores purchased from Invitrogen or other suppliers including but not limited to Alexa Fluor® 647, Cy3, Cy5, and ULight dyes. Terbium antibodies are compatible with fluorescein or GFP.
Ordering Information
| Product | Label | Trial kit (5ug) | Trial kit (25ug) | 25ug Ab only | 1mg Ab only |
| LanthaScreen® Eu-anti-DYKDDDDK | Europium | PV6034 | PV6026 | PV6027 | |
| LanthaScreen® Eu-anti-GST | Europium | PV5594 | PV5595 | ||
| LanthaScreen® Eu -anti-His Tag | Europium | PV5596 | PV5597 | ||
| LanthaScreen® Tb-anti-GST | Terbium | PV3550 | PV3551 | ||
| LanthaScreen® Elite Tb-anti-His Tag | Terbium | PV5863 | PV5895 | ||
| LanthaScreen® Tb-anti-GFP Antibody | Terbium | A13391 | A13392 |
LanthaScreen® Secondary Antibodies
Our secondary anti-species antibodies bind to their corresponding species IgG with low nanomolar apparent Kds, and show negligible cross-reactivity with other species. These antibodies can be used to rapidly develop assays when an anti-target antibody is already available. The labeled anti-species antibody will bind to the anti-target IgG completing the TR-FRET pair for easy assay development.
Our products are available as kits or stand alone antibodies. Kits contain a positive control peptide for the antibody, and 100 mL of TR-FRET dilution buffer (PV3574) as a dilutent for the antibody during biochemical assay development.
We are continuously expanding our portfolio of Europium-labeled secondary antibodies. To gain early access to these targets, please email us at discoverysciences@invitrogen.com.
Our products are available as kits or stand alone antibodies. Kits contain a positive control peptide for the antibody, and 100 mL of TR-FRET dilution buffer (PV3574) as a dilutent for the antibody during biochemical assay development.
We are continuously expanding our portfolio of Europium-labeled secondary antibodies. To gain early access to these targets, please email us at discoverysciences@invitrogen.com.
Ordering Information
LanthaScreen® Streptavidin and Biotin
Streptavidin and anti-Biotin antibody are reagents that can be used to easily label biotinylated biomolecules with lanthanide chelate for use in TR-FRET assays. Although the chelate donor can be directly attached to a biomolecule, these reagents offer a means to quickly mediate this association.
Our products are available as kits or stand alone antibodies. Trial kits contain 100mL of TR-FRET dilution buffer (PV3574), which is recommended for dilution, and a positive control peptide.
Our products are available as kits or stand alone antibodies. Trial kits contain 100mL of TR-FRET dilution buffer (PV3574), which is recommended for dilution, and a positive control peptide.
Ordering Information
| Product | Label | Trial kit (10ug) | Trial kit (50ug) | 50ug Streptavidin only | 1mg Streptavidin only |
| LanthaScreen® Eu-Streptavidin | Europium | PV6030 | PV5899 | PV6025 | |
| LanthaScreen® Tb-Streptavidin | Terbium | PV3965 | PV3965 | PV3966 |
| Product | Label | Trial kit (5ug) | Trial kit (25ug) | 25ug Ab only | 1mg Ab only |
| LanthaScreen® Eu-anti-Biotin | Europium | PV6033 | PV5901 | PV5900 | |
| LanthaScreen® Tb-anti-Biotin | Terbium | PV4749 | PV4745 | PV4746 |
LanthaScreen® Reactive Chelate Labeling
Invitrogen offers do-it-yourself reagents or a custom service for labeling of your antibody or protein of interest. Reagents include amine-reactive, isothiocyanate-functionalized LanthaScreen® chelates and thiol-reactive, maleimide-functionalized LanthaScreen® terbium chelates for development of TR-FRET assays in-house. Europium chelate reagents are available on a custom basis. Contact us at discoverysciences@invitrogen.com to gain access.
Alternatively, we can custom label your antibody (or other protein) with either a europium or terbium chelate. Let us know what antibody you would like us to label (vendor information) or supply antibody in a buffer without carrier protein (i.e. BSA), and we will label it for you.
Contact us at discoverysciences@invitrogen.com to learn more about custom labeling.
Alternatively, we can custom label your antibody (or other protein) with either a europium or terbium chelate. Let us know what antibody you would like us to label (vendor information) or supply antibody in a buffer without carrier protein (i.e. BSA), and we will label it for you.
Contact us at discoverysciences@invitrogen.com to learn more about custom labeling.
More Information
Related Products
- LanthaScreen® Kinase Activity Assays
- LanthaScreen® Eu Kinase Binding Assays
- Adapta® Universal Kinase Assays
- LanthaScreen® Ubiquitinylation Assay Tools
