LanthaScreen® Reagent Table
Our LanthaScreen® kinase activity assay toolbox of "mix and match" assay reagents is designed to enable rapid assay configuration. Our portfolio of phospho-specific antibodies, matched fluorescent peptide substrates and in-house produced kinases have been tested in >200 different kinase assays to date. |
Phospho-specific Antibodies with Peptide Substrates
To facilitate in-house kinase assay configuration and screening, we have developed a selection of Tb-labeled phospho-specific antibodies, which may be accessed in small scale and screening quantities. Matched and validated substrates are available for kinase assay configuration.
These antibodies may also be available with Europium labels, contact us at discoverysciences@invitrogen.com, for more information.
These antibodies may also be available with Europium labels, contact us at discoverysciences@invitrogen.com, for more information.
Tyrosine Kinase Assays
| SKU | Product Catalog | Size |
|---|---|---|
| PV5692 | LanthaScreen® Eu-PY20 Antibody Kit | 25μg |
| PV5696 | LanthaScreen® Eu-PY20 Antibody Kit | 1mg |
| PV5693 | Alexa Fluor® 647-Poly GT | 1 nmol |
| PV5836 | Alexa Fluor® 647-Poly GT | 10 nmol |
| PV3552 | LanthaScreen® Tb-PY20 Antibody Kit | 25μg |
| PV3553 | LanthaScreen® Tb-PY20 Antibody Kit | 1mg |
| PV3610 | Fluorescein-Poly GT, 30 μM | 1mL |
| PV3611 | Fluorescein-Poly GAT, 30 μM | 1mL |
Serine/Threonine Kinase Assays
| SKU | Product Catalog | Size |
|---|---|---|
| PV3560 | LanthaScreen® Tb-pSer (PKC) Antibody Kit | 25μg |
| PV3561 | LanthaScreen® Tb-pSer (PKC) Antibody Kit | 1mg |
| PV3506 | Fluorescein-PKC Substrate | 1mg |
| PV3562 | LanthaScreen® Tb-IKBa [pSer32] Antibody Kit | 25μg |
| PV3563 | LanthaScreen® Tb-IKBa [pSer32] Antibody Kit | 1mg |
| PV3507 | Fluorscein-IKK substrate | 1mg |
| PV3564 | LanthaScreen® Tb-pCrosstide Antibody Kit | 25μg |
| PV3565 | LanthaScreen® Tb-pCrosstide Antibody Kit | 1mg |
| PV3509 | Fluorescein-Crosstide Substrate | 1mg |
| PV3566 | LanthaScreen® Tb-CREB pSer133 Antibody Kit | 25μg |
| PV3508 | Fluorescein-CREBtide Substrate, 1 mg/mL | 1mg |
| PV4899 | LanthaScreen® Tb-pERM (pLRRKtide) Antibody Kit | 25μg |
| PV4900 | LanthaScreen® Tb-pERM (pLRRKtide) Antibody Kit | 1mg |
| PV4901 | Fluorescein-ERM (LRRKtide) Substrate | 1mg |
| PV5130 | LanthaScreen® Tb-anti-phospho-p53 [pSer15] Antibody Kit | 25μg |
| PV5131 | LanthaScreen® Tb-anti-phospho-p53 [pSer15] Antibody Kit | 1mg |
| PV5132 | Fluorescein-p53 [Ser15] Substrate | 1mg |
Phospho-specific Antibodies with Physiological Protein Substrates
As an extension to our peptide-based substrates for LanthaScreen® kinase activity assays, we now offer physiological protein substrates that are directly labeled with a green fluorescent protein (GFP) acceptor fluorophore. Pairing the GFP-labeled substrate with a corresponding terbium (Tb)-labeled phosphospecific antibody allows detection of the phosphorylated product.
Ordering Information
| SKU | Product Catalog | Size |
|---|---|---|
| PV4451 | LanthaScreen® Tb-ATF2 [pSer71] Antibody Kit | 25μg |
| PV4452 | LanthaScreen® Tb-ATF2 [pSer71] Antibody Kit | 1mg |
| PV4445 | GFP-ATF2 (19-96) | 20 nmol |
| PV4453 | LanthaScreen® Tb-c-Jun [pSer73] Antibody Kit | 25μg |
| PV4454 | LanthaScreen® Tb-c-Jun [pSer73] Antibody Kit | 1mg |
| PV4446 | GFP-c-jun (1-179) | 20 nmol |
| PV4757 | LanthaScreen® Tb-4E-BP1 [pThr46] Antibody Kit | 25μg |
| PV4758 | LanthaScreen® Tb-4E-BP1 (pThr46) Antibody Kit | 1mg |
| PV4759 | GFP-4E-BP1 | 20 nmol |
| PV4810 | LanthaScreen® Tb-eIF2a [pSer52] Antibody Kit | 25μg |
| PV4811 | LanthaScreen® Tb-eIF2a [pSer52] Antibody Kit | 1mg |
| PV4809 | GFP-eIF2a | 20 nmol |
| PV4813 | LanthaScreen® Tb-MAP2K1 [pSer217/221] Antibody Kit | 25μg |
| PV4814 | LanthaScreen® Tb-MAP2K1 [pSer217/221] Antibody Kit | 1mg |
| PV4812 | Fluorescein-MEK1 | 20 nmol |
| PV4844 | LanthScreen® Tb-anti-STAT1 [pTyr701] Antibody Kit | 25μg |
| PV4845 | LanthScreen® Tb-anti-STAT1 [pTyr701] Antibody Kit | 1mg |
| PV5211 | GFP-STAT1 | 20 nmol |
| PV4846 | LanthaScreen® Tb-STAT3 [pTyr705] Antibody Kit | 25μg |
| PV4847 | LanthaScreen® Tb-STAT3 [pTyr705] Antibody Kit | 1mg |
| PV5212 | GFP-STAT3 | 20 nmol |
Buffers and Other Supplemental Assay Reagents
To complete our offering of antibodies and substrates for the development of TR-FRET kinase assays, we offer a selection of validated kinase buffers and other assay reagents.
Ordering Information
| SKU | Product Catalog | Size |
|---|---|---|
| PV3574 | TR-FRET Dilution Buffer | 100mL |
| PV3189 | 5X Kinase Buffer A | 4mL |
| PV5125 | 5X Kinase Buffer Q | 4mL |
| PV5101 | 5X Kinase Buffer R | 4mL |
| PV5213 | 5X Kinase Buffer S | 4mL |
| PV4794 | 1X mTOR Assay Buffer | 20mL |
| P2825 | Kinase Quench Buffer | 1mL |
| PV3227 | 10 mM ATP | 500μL |
| P2325 | 1M DTT | 1mL |
More Information
Generic LanthaScreen® Reagents
