Biochemical Kinase Assays
 | Life Technologies offers you the broadest set of biochemical fluorescent assays to address high-throughput screening, hit confirmation and lead optimization programs. All Life Technologies kinase assay technologies are homogenous, non-radioactive, and highly miniaturizable – and are available as kits, custom or bulk reagents. For quality kinase profiling data on rapid timelines, choose SelectScreen®, the industry’s most utilized kinase profiling service. To compile the components needed to make the right biochemical kinase assay for your research, use our new Drug Discovery Assay Maker™ web tool. |
Choose the right biochemical HTS kinase assay for your research
| Compatible with fluorescently labeled whole protein substrates | Ideal for kinetic studies including on/off rates or for kinases with no known substrate | Validated specifically for lipid kinases; can use with unmodified peptide or lipid substrates | Primary assay for SelectScreen® Kinase Profiling Service | ||||
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| Technology basis |
Phospho-antibody TR-FRET | Tracer-based TR-FRET | ADP detection by TR-FRET | FRET peptide phosphorylation | |||
Application |
Primary screening | Unactivated or low-activity kinase target screening | Primary and secondary screening | Secondary screening, hit validation, and profiling | |||
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Number of kinases validated |
200 | 239 | 20 | 233 | |||
| Readout | Endpoint | Endpoint or Kinetic | Endpoint | Endpoint | |||
| Total assay volume | 20 µL | 15 µL | 15 µL | 20 µL | |||
| ATP concentration range | Tested up to 1 mM | ATP not used | Tested from 10 to 100 µM | Tested up to 1 mM | |||
| Substrate type | Peptide or protein | No substrate needed | Peptide, protein or lipid | Peptide | |||
| Substrate concentration range | 100 nM - 1 µM | No substrate needed | Flexible; must be greater than ATP concentration | 2 µM only | |||
| Plate signal stability | Up to 18 hours | Up to 24 hours | Up to 18 hours | 4 - 18 hours (kit-dependent) | |||
| Cell lysate compatible |  |
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| Overcoming interference | Time-resolved readout allows read after interfering signals have decayed | Time-resolved readout allows read after interfering signals have decayed; use of epitope-tag ensures no contaminating kinase is detected | Time-resolved readout allows read after interfering signals have decayed; red Alexa Fluor® dye as acceptor further enhances ability to overcome interference | Preread at 445/520 nm | |||
| Cost per well | $ - $$ | $ - $$ | $$ - $$$ | $$ | |||
| Use in SelectScreen® Kinase Profiling Services | |
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Optimize with multiple high-throughput assay formats
Life Technologies offers four high-throughput assay technologies for your biochemical kinase research.
- LanthaScreen™ Eu Kinase Binding Assays - Obtain sensitive detection of kinase inhibitors, including allosterics; interrogate active and non-activated kinases; track slow-binding inhibitors in real time, and compare inhibitor on-off rates with this robust assay technology.
- LanthaScreen™ Kinase Activity Assays – A powerful toolbox for TR-FRET kinase activity assay development – choose exactly the reagents you need and acquire high quality data with minimal assay interference.
- Adapta™ Universal Kinase Assays – A universal TR-FRET assay suitable for all kinases; including difficult-to-assay targets such as lipid kinases.
- Z´-LYTE® Kinase Assay – An ideal assay for secondary screening, hit validation and profiling, Z’-LYTE is the primary assay technology utilized in our SelectScreen® Kinase Profiling Service.
Lower Throughput Assay Technologies
- Omnia® Kinase Assays - Use for mechanistic studies. Simple mix-and-read fluorescence-based assay allows measurements in real time (kinetics).
- NDP Sensor - A universal, real-time kinetic kinase assay platform at a very low price per well
