FluxOR™ Potassium Ion Channel Assay
Figure 1. Principle of the FluxOR™ assay. Basal fluorescence from cells loaded with the FluxOR™ dye is low, as shown in the left panel, until potassium channels are stimulated. When thallium is added to the assay with the stimulus, the thallium flows down its concentration gradient into the cells, activating the dye as shown in the right panel.
Figure 3. FluxOR™ potassium ion channel assay signatures of Kv7.2 and Kv 7.3, expressed alone (left panel) or together (right panel). Cells were transduced with 5% vol/vol of each construct alone or together and subjected to the FluxOR™ assay. A control virus (null BacMam) was used to show the background level of activity in the assay (lowest trace). The stimulus was injected 1:5 vol/vol to yield a final added potassium concentration of 10 mM, and a final added thallium concentration of 2 mM. Kv7.2 and Kv7.3 must be co-expressed and properly fold into a tetramer in order to obtain potassium ion flux detectable with the FluxOR™ assay.
Assay ready Ion Channel Reagents
View our collection of BacMam Ion Channel Reagents