Baculosomes® Plus P450 Proteins
Drug metabolizing enzymes (DME) are a diverse group of enzymes located primarily in the liver. They metabolize vast array of xenobiotic compounds including drugs, environmental pollutants, and endogenous compounds such as steroids and prostaglandins.
Cytochrome P450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells infected with recombinant baculovirus containing cDNA for a human P450 isozyme, human NADPH-P450 reductase, and in some cases human cytochrome b5. P450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes (HLMs), for targeted studies of P450 isoforms, because only one P450 is expressed. This prevents interfering metabolism by other P450s or other classes of DMEs.
Compound rankings based on metabolism and inhibition profiles observed with P450 BACULOSOMES® Plus Reagents are very similar to those seen with HLMs for most compounds tested. Activity and metabolic rates of the P450 BACULOSOMES® Plus Reagents with most substrates, however, is significantly higher than those observed from preparations of HLMs.