CYP1A1 Induction Assay
| The GeneBLAzer® CYP1A1-bla LS-180 cell line allows measurement of CYP1A1 induction via a beta-lactamase reporter gene (Figure 1). The beta-lactamase (bla) gene is inserted within the CYP1A1 promoter, and delivers a readout for CYP1A1 transactivation. Ligands such as TCDD and 3-methylcholanthrene bind the aryl hydrocarbon receptor (AhR) in the cytoplasm, which leads to translocation of the AhR-ligand complex to the nucleus where it forms a heterodimer with AhR nuclear translocator (Arnt). This complex can then bind the xenobiotic response element (XRE) in the CYP1A1 promoter, enhancing CYP1A1 expression. |
Figure 1. Dose response of CYP1A1 inducers in the CYP1A1-bla LS180 assay. Cells were treated with various concentrations of 3-methylcholanthrene or 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) for 16 hours and then assayed for beta-lactamase activity.
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