BMG LABTECH
 | The PHERAstar, PHERAstar Plus and Omega microplate readers have received the LanthaScreen® Certified Plus designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument setup and assay performance.
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Test your plate reader set-up before using LanthaScreen® Terbium and Europium assays
These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.
PHERAstar/PHERAstar Plus Microplate Readers
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Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Omnia® | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FI (kinetic) | FP | FRET |
| Setup Guide |  | Reader not compatible | | | | |
| Application Note | - | - | - | - | View | - |
| Poster | - | - | - | - | View | - |
| Excitation Filter | 337 | 337 | 360 | 540 | 400 | |
| Emission 1 Filter | 665 | 520 | 485 | 590 | 440 | |
| Emission 2 Filter | 620 | 490 | 590 | 520 | ||
| Dichroic Mirror | Included | Included | Included | |||
| Optic Module | HTRF® | LanthaScreen® | Red FP |
Omega Microplate Reader
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Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Omnia® | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FI (kinetic) | FP | FRET |
| Setup Guide | | | | | Reader not compatible | |
| Application Note | - | - | - | - | - | - |
| Poster | - | - | - | - | - | - |
| Excitation Filter | 337 | 400 | 337 | 360 | 400 | |
| Emission 1 Filter | 665 | 440 | 520 | 485 | 440 | |
| Emission 2 Filter | 620 | 520 | 490 | 520 |
OPTIMA Microplate Reader
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Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Omnia® | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FI (kinetic) | FP | FRET |
| Setup Guide | Reader not compatible | | Reader not compatible | | Reader not compatible | |
| Application Note | - | - | - | - | - | - |
| Poster | - | - | - | - | - | - |
| Excitation Filter | 400 | 360 | 400 | |||
| Emission 1 Filter | 440 | 485 | 440 | |||
| Emission 2 Filter | 520 | 520 |
* Filters shown as center wavelength (nm); Contact BMG LABTECH for information on ordering optics modules & filters suitable with the microplate readers listed above.
HTRF® is a registered trademark of Cisbio Assays
