Research Tools for Influenza Detection

Influenza detection requires several distinct steps, including isolation of the viral RNA, reverse transcription of the viral RNA to cDNA, and realtime PCR-based amplification and detection of known subtype sequences. Life Technologies supplies high-performing products for each step in this process.

RNA isolation and amplification reagents have been optimized for use on Applied Biosystems instrumentation; superior instrument quality and on-site certified Field Service Engineer Support minimize down time and help ensure that a maximum number of samples can be processed per day. Real-time PCR-based detection of known influenza strains using sequence-specific dual-labeled (TaqMan® chemistry) probes is recommended by the CDC for more accurate results compared to antibody-based methods. Life Technologies offers a portfolio of Research Use Only real-time PCR products for every step of the influenza detection process.

Step-by-Step Guide to Influenza Detection

The influenza virus is RNA-based; therefore, in order to detect the virus, RNA must be extracted and converted to cDNA. High yields of viral RNA may be obtained from respiratory fluids such as nasal swabs or throat swabs, using products available from Applied Biosystems and Invitrogen.

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