Testing Instruments

Applied Biosystems® instruments have a proud history of leading the biotechnology industry. Inventors of fluorescence-based automated sequencing technology as well as real-time PCR science, we are now among the world’s leading companies pioneering next-generation sequencing as well as numerous other innovations. Unless otherwise noted, all instruments are for research use only, not for use in diagnostic procedures.

Sequencing instruments

  3500 Genetic Analyzer 5500xL Genetic Analyzer
Instrument/ Capacity

3500 Series Genetic Analyzers

One of the most user-friendly genetic analyzers on the market.
The Applied Biosystems® 3500 Dx is CE-IVD labeled in accordance with the IVD Directive.

  • Snap-in-and-run, preformulated consumables packaging
  • Auditable tracking of instrument performance and consumables usage

5500 Series Genetic Analyzers

Maximum productivity and industry-leading accuracy, designed with flexibility and cost efficiency in mind.

  • Pay-per-lane sequencing means you only pay for the lanes you run
  • Run different applications (fragment, paired-end, mate-paired) on one FlowChip

Personal Genome Machine™ (PGM™) Sequencer

Ion Torrent™ semiconductor sequencing rapidly generates large quantities of high-quality data

  • Produces sequence in less than 2 hours (up to 200 bp reads)
  • Delivers >99.97% accuracy

Real-time PCR instruments

 
Instrument/ Capacity

StepOnePlus™
96-well plates, 0.1 mL tubes

StepOne™
48-well plates, 0.1 mL tubes

7500 Fast
7500 Fast Dx

96-well optical Fast plates.

7500Fast
96 well optical plates or 0.2 mL tubes.

ViiA™ 7
96-well
384-well
TaqMan® Array Microfluidic Cards

ViiA™ 7Dx

QuantStudio™ 12K Flex Real-Time PCR System
96-well
(10–100 μL reactions),
Fast 96-well
(15–30 μL reactions)
384-well
(5–20 μL reactions)
TaqMan® Array Cards
(~1 μL reactions)
OpenArray® Plates
(33 nL reactions)

Temperature Uniformity ± 0.5°C within 30 seconds of clock start ± 0.5°C within 30 seconds of clock start ±0.5°C, within 30 sec of reaching temperature set point ±0.25°C (±0.75°C for OpenArray® block)
Fast block Yes

7500 Fast & 7500 Fast Dx
Yes

7500 & 7500 for HID
No

Yes
(96-well and 384-well)
Yes—96-well
Performance Specifications
Demonstrated Sensitivity 10 copies 10 copies Down to 1 copy Down to 1 copy
Dynamic Range 9 logs of linear dynamic range 5 logs guaranteed, 9 logs demonstrated Up to 9 logs of linear dynamic range Up to 9 logs of linear dynamic range (up to 7 logs for OpenArray® block)
Instrument Specifications
Size
(W x D x H)
W 23.6 cm
D 51.2 cm
H 42.7 cm
W 34 cm
D 45 cm
H 49 cm
W 53.3 cm
D 63.5 cm
H 64.5 cm
W 50.5 cm
D 67.2 cm
H 73.8 cm
Multiplex Quantitation Yes Yes Yes Yes (on all non-OpenArray® blocks, duplex on OpenArray®)

Flow Cytometry

  3500 Genetic Analyzer
Instrument/ Capacity

Attune® Acoustic Focusing Cytometer

Acoustic focusing technology delivers accurate data at all flow rates and sample sizes.

  • Exceptional separation of signal peaks offers more precision
  • Better rare-event detection than traditional methods