Pre-filled Wave CellBag

wave bioreactor cell culture
 The Cellbag® Bioreactor from Wave Biotech is now available pre-filled with GIBCO® media.

  • Ready to ship - media formulations include Sf-900II and FreeStyle™ 293
  • Convenient - Cellbag® chambers are filled and ready to use
  • Customizable - you choose the GIBCO® medium formulation and volume.

GIBCO® can fill any 10 or 20-L Wave O-series Cellbag with the media of  your choice. For optimal cell growth and production, choose the Cellbag® disposable bag size, volume, and GIBCO® medium formulation that's right for you.

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Gibco Bioreactors Chart

GIBCO® serum-free medium and Wave disposable bioreactors deliver superior insect cell growth and robust protein expression compared to a glass-lined bioreactor.  

(Panel A) Growth and viability of Sf9 cells grown in Sf-900™ III medium in Wave Cellbag® bioreactor chambers and CelliGen Plus® bioreactors.  Preadapted Sf9 cells were used to inoculate a Wave Cellbag® Bioreactor Chamber and a CelliGen Plus® Bioreactor at a seeding density of 4 × 105 cells/ml.  Cell number and viability were monitored daily for 8 days.  Each data point represents a single sample. 

(Panel B) Sf9 cells were grown under conditions identical to those described in A, with introduction of a recombinant baculovirus containing the lacZ gene at day 2 post-inoculation (Sf9 density ~2 x 106 cells/ml, baculovirus MOI ~5).  Cell number and viability were monitored daily for 8 days.  Each data point represents a single sample. 

(Panel C) Cell samples (0.5 ml) were taken daily for 4 days post-infection from the Wave Cellbag® bioreactor chamber and a CelliGen Plus® bioreactor described in Figure 2B and assayed for ß-galactosidase activity.