|The RNA UltraSense™ One-Step qRT-PCR System is specifically designed for amplification and real-time detection of RNA viruses and ultralow-abundance transcripts. The optimized system combines SuperScript® III reverse transcriptase and Platinum® Taq DNA polymerase to provide the most sensitive and specific results over a broad dynamic range. The concentrated 5X qRT-PCR Reaction mix allows samples to be up to 70% of the reaction mixture volume for greater flexibility with low-concentration, high-volume RNA samples.|
RNA UltraSense™ One-Step qRT-PCR System
- Highest sensitivity for best detection of low copy viruses
- Rapid optimization for difficult RNA secondary structures
- Powered by SuperScript™ III RT performance and Platinum® Taq accuracy
Viral RNA can be extremely difficult to amplify due to the presence of significant secondary structure. The RNA UltraSense™ One-Step qRT-PCR System includes a proprietary enzyme mixture that improves performance by promoting primer-template interactions with difficult samples. In addition, the high thermostability of SuperScript® III RT allows cDNA synthesis at temperatures up to 50°C, increasing the success rate with RNA rich in secondary structure.
Figure 1. Amplification of single-stranded RNA virus.
Newcastle that exhibits considerable secondary structure. qRT-PCR was performed on 10-fold serial dilutions of RNA. The RNA UltraSense™ One-Step qRT-PCR System provided clean amplification with 90% efficiency.