BioDefense - Support Products
| Gene Acquisition | |
| Product | Benefit |
| Ultimate™ ORF Clones | Immediate access to clones saves time and resources. Provides ultimate downstream flexibility for expression and analysis. |
| Gene-specific custom primers | Synthetic oligonucleotides are made to order. |
| pENTR/D-TOPO® Cloning kit | The fastest method of entry into Gateway® Technology. |
| Expression Systems | |
| Product | Benefit |
| Expressway™ Plus In Vitro Protein Synthesis System | For fast, easy, cost-effective protein expression. Offers greater protein activity than the competition and is compatible with Gateway® Technology. |
| Champion™ pET Expression Systems | High-level expression in E. coli using familiar methods. System is scalable with multiple cloning, purification, and detection options, including TOPO® and Gateway® Technologies. |
| LR Clonase™ Enzyme Mix | For efficient in vitro recombination between attL-containing entry vectors and attR-containing destination vectors in Gateway® Technology. |
| BaculoDirect™ Expression Kit | Provides the fastest and easiest method of generating recombinant baculovirus. Gateway® Technology eliminates time-consuming steps and allows for extreme time savings by producing high levels of soluble protein; insect cells grow as suspension cultures and do not require CO2. |
| DES®-Inducible Kit with pCoBlast | Provides for regulated stable expression in Drosophila S2 cells. Also allows for multiple purification and detection options. |
| DES®-Blasticidin Support Kit | Complete kit for the fastest selection of stable cell lines. |
| FreeStyle™ 293 Expression System | Provides the easiest method for optimized and scalable high-level transient mammalian expression in suspension 293 cells. |
| Lipofectamine™ 2000 Reagent | Allows for high-efficiency transfection in a broad range of cell lines. |
| ViraPower™ Lentiviral Gateway® Expression Kit | The only method to transduce both non-dividing and dividing cells for high-level stable expression experiments. Allows efficient generation of virus using Gateway® Technology. Using the same virus on any cell type, viral delivery is ideal for difficult-to-transfect cells. |
| ViraPower™ Adenoviral Gateway® Expression Kit | With high-level transient gene expression in any cell type and the most efficient generation of adenoviral clones, you save time. |
| Flp-In™ System | Offers a fast way to generate stable cell lines while eliminating the variation seen with random integration. With the Flp-In™ System your gene will be expressed from the same site in every cell. Direct comparison between multiple genes is now a possibility. |
pVAX™200-DEST Vector System | Highest Level Expressing Minimal-Sequence Vector, Incorporating Gateway® Technology for Fast, Efficient Construction |
| Tissue Culture Cell Lines and Media | |
| Product | Benefit |
| Flp-In™ CHO Cell Line | Contains a confirmed single, high-level expressing FRT site already integrated into a CHO cell line. |
| BL21-Star™ E. coli | Provides a very high-level T7 expression host. |
| BL21-AI™ E. coli | Provides high-level, arabinose-inducible T7 expression host. |
| GIBCO® Advanced D-MEM and Advanced MEM | Provides for up to 80% reduction in serum needs, decreased variability, quality and technical expertise. Directly replaces D-MEM. |
| GIBCO® OptiPro™ SFM and VP SFM Animal Origin-Free | Serum-free media optimized for growth of kidney-derived cell lines and high level expression of virus and recombinant protein. |
| GIBCO® CD CHO Medium | Chemically defined protein-free median used for growth and production of recombinant proteins in suspension culture, free of animal-derived components. |
| GIBCO® CHO-S cells, SFM-adapted to GIBCO® CD CHO Medium | Pre-adapted CHO cells optimized for suspension culture applications, eliminate the need for adaptation to serum-free conditions, ideal for high-density and bioreactor applications. |
| GIBCO® Sf900 II SFM | Reduces cost, complexity, and variables with protein-free insect cell culture medium optimized for the growth and maintenance of Sf9 and Sf21 cells when adapting cultures to SF and/or suspension cultures. |
| GIBCO® Sf9 Cell Line and SP21 Cell Line | Clonal isolate adapted to serum-free suspension culture in Sf-900 II SFM saves significant time and expense. |
| GIBCO® Express Five™ SFM | Protein-free medium for the growth and maintenance of cells used for the Baculovirus Expression Vector Systems (BEVS) for adherent and suspension culture. Allows for large-scale production of recombinant protein expressed by BEVS. |
| GIBCO® 293 II and CD 293 Medium; 293-F and 293-II cells, SFM-adapted | Serum-free defined media for use in HEK 293 cells foroptimum expression and yield. Pre-adapted 293 cells eliminate the need for adaptation to serum-free conditions. |
| GIBCO® GlutaMAX™ Media | Reduces glutamine degradation and ammonia toxicity. |
| GIBCO® OptiMEM® I Media | Highly useful for a wide range of fibroblasts and epithelial cells. Allows for up to 80% reduction in serum needs, decreasing variability. |
| Mimic™ Sf9 Cells | Provides production of mammalian-like glycosylated proteins in insect cells. |
| Nucleic Acids | |
| Product | Benefit |
| Micro-to-Midi Total RNA Purification System | Isolate the highest quality total RNA, from 100 cells to 200 mg of tissue. |
| Custom Oligonucleotides | Quality customization from the leading provider of custom oligonucleotides gives you the best results every time. |
| 0.17-1.77 Kb RNA Ladder | For sizing single-stranded RNA in denaturing gels. |
| SuperScript™ III Reverse Transcriptase | Generates the highest yield of cDNA during first-strand synthesis. |
| E. coli DNA Polymerase I | Optimized polymerase is used in second-strand cDNA synthesis. |
| RNA UltraSense™ One-Step Quantitative RT-PCR System | For amplification of RNA viruses and ultra-low abundance transcripts with SuperScript™ III RT and Platinum® Taq DNA Polymerase |
| Microarrays and Real-Time PCR | |
| Product | Benefit |
| SuperScript™ Indirect cDNA Array Labeling Kit | Comes complete and optimized with all reagents (except fluorescent dyes). |
| Human Cot-1 DNA® | Gold standard for blocking non-specific background during hybridization (human genes). |
| Platinum® Quantitative RT-PCR Thermoscript™ One-Step System | This enzyme mix with optimized combination of high thermostability and RNase H- gives the highest yield for difficult RNA templates. |
| Custom LUX™ Fluorogenic Primers (for detection in RT-PCR or qPCR) | Comparable to dual-labeled probes at half the cost. |
| SuperScript™ First-Strand Synthesis System for RT-PCR | System is optimized for high-performance first-strand synthesis using SuperScript™ RT. |
| Protein Analysis | |
| Product | Benefit |
| ZOOM® IPGRunner™ 2D Gel Electrophoresis Cassettes | Ready-to-use self-contained electrophoresis cassettes. Each cassette runs six strips, with two cassettes fitting in the ZOOM® IPGRunner™ unit. |
| ZOOM® IPGRunner™ Strips and Ampholytes | Each ZOOM® strip is labeled with a unique identifying number, pH range, and orientation marks for easy usage. |
| NuPAGE® Bis-Tris ZOOM® Gels and Buffers | Neutral pH Bis-Tris gels provide the sharpest spots with the least protein modification for the most accurate results in mass spectromity analysis of proteins. |
| SimplyBlue™ SafeStain | A fast, sensitive, environmentally-safe redundant Coomassie® G-250 stain that detects nanogram levels of protein and is mass spectrometry-compatible. |
| SilverQuest™ Silver Staining Kit | A fast, ultra-sensitive silver stain which is formulated to provide minimal protein modification for optimal mass spectrometry results. |
| WesternBreeze® Immunodetection Kits | Optimized with everything you need for a successful western blot while reducing your transfer optimization time. Available for chromogenic and chemiluminescent detection. |
| MagicMark™ Western Protein Standard | Allows for easy molecular weight estimation directly on western blots. Each band contains an IgG binding site so you can detect the standard with the same antibody-conjugates used to detect your sample. |
| Anti-Epitope Antibodies | Provides purified mouse monoclonal antibodies which detect recombinant proteins containing the epitope tag. |
| Custom Antibody Services | Create monospecific polyclonals against peptides or whole protein. We offer a wide range of antibody services, and with EvoTrack™ Online, you can manage your projects on the web easily and securely. |
| Custom Peptide Services | Based on your specifications, we synthesize the peptide sequence of your choice in state-of-the art facilities. Several scales, purities and modifications available to ensure you get exactly what you want. |
