BioDefense - Support Products
Gene Acquisition
Product Benefit
Ultimate™ ORF Clones Immediate access to clones saves time and resources. Provides ultimate downstream flexibility for expression and analysis.
Gene-specific custom primers Synthetic oligonucleotides are made to order.
pENTR/D-TOPO® Cloning kit The fastest method of entry into Gateway® Technology.

 

Expression Systems
Product Benefit
Expressway™ Plus In Vitro Protein Synthesis System For fast, easy, cost-effective protein expression. Offers greater protein activity than the competition and is compatible with Gateway® Technology.
Champion™ pET Expression Systems High-level expression in E. coli using familiar methods. System is scalable with multiple cloning, purification, and detection options, including TOPO® and Gateway® Technologies.
LR Clonase™ Enzyme Mix For efficient in vitro recombination between attL-containing entry vectors and attR-containing destination vectors in Gateway® Technology.
BaculoDirect™ Expression Kit Provides the fastest and easiest method of generating recombinant baculovirus. Gateway® Technology eliminates time-consuming steps and allows for extreme time savings by producing high levels of soluble protein; insect cells grow as suspension cultures and do not require CO2.
DES®-Inducible Kit with pCoBlast Provides for regulated stable expression in Drosophila S2 cells. Also allows for multiple purification and detection options.
DES®-Blasticidin Support Kit Complete kit for the fastest selection of stable cell lines.
FreeStyle™ 293 Expression System Provides the easiest method for optimized and scalable high-level transient mammalian expression in suspension 293 cells.
Lipofectamine™ 2000 Reagent Allows for high-efficiency transfection in a broad range of cell lines.
ViraPower™ Lentiviral Gateway® Expression Kit The only method to transduce both non-dividing and dividing cells for high-level stable expression experiments. Allows efficient generation of virus using Gateway® Technology. Using the same virus on any cell type, viral delivery is ideal for difficult-to-transfect cells.
ViraPower™ Adenoviral Gateway® Expression Kit With high-level transient gene expression in any cell type and the most efficient generation of adenoviral clones, you save time.
Flp-In™ System Offers a fast way to generate stable cell lines while eliminating the variation seen with random integration. With the Flp-In™ System your gene will be expressed from the same site in every cell. Direct comparison between multiple genes is now a possibility.
pVAX™200-DEST Vector System Highest Level Expressing Minimal-Sequence Vector, Incorporating Gateway® Technology for Fast, Efficient Construction

 

Tissue Culture Cell Lines and Media
Product Benefit
Flp-In™ CHO Cell Line Contains a confirmed single, high-level expressing FRT site already integrated into a CHO cell line.
BL21-Star™ E. coli Provides a very high-level T7 expression host.
BL21-AI™ E. coli Provides high-level, arabinose-inducible T7 expression host.
GIBCO® Advanced D-MEM and Advanced MEM Provides for up to 80% reduction in serum needs, decreased variability, quality and technical expertise. Directly replaces D-MEM.
GIBCO® OptiPro™ SFM and VP SFM Animal Origin-Free Serum-free media optimized for growth of kidney-derived cell lines and high level expression of virus and recombinant protein.
GIBCO® CD CHO Medium Chemically defined protein-free median used for growth and production of recombinant proteins in suspension culture, free of animal-derived components.
GIBCO® CHO-S cells, SFM-adapted to GIBCO® CD CHO Medium Pre-adapted CHO cells optimized for suspension culture applications, eliminate the need for adaptation to serum-free conditions, ideal for high-density and bioreactor applications.
GIBCO® Sf900 II SFM Reduces cost, complexity, and variables with protein-free insect cell culture medium optimized for the growth and maintenance of Sf9 and Sf21 cells when adapting cultures to SF and/or suspension cultures.
GIBCO® Sf9 Cell Line and SP21 Cell Line Clonal isolate adapted to serum-free suspension culture in Sf-900 II SFM saves significant time and expense.
GIBCO® Express Five™ SFM Protein-free medium for the growth and maintenance of cells used for the Baculovirus Expression Vector Systems (BEVS) for adherent and suspension culture. Allows for large-scale production of recombinant protein expressed by BEVS.
GIBCO® 293 II and CD 293 Medium; 293-F and 293-II cells, SFM-adapted Serum-free defined media for use in HEK 293 cells foroptimum expression and yield. Pre-adapted 293 cells eliminate the need for adaptation to serum-free conditions.
GIBCO® GlutaMAX™ Media Reduces glutamine degradation and ammonia toxicity.
GIBCO® OptiMEM® I Media Highly useful for a wide range of fibroblasts and epithelial cells. Allows for up to 80% reduction in serum needs, decreasing variability.
Mimic™ Sf9 Cells Provides production of mammalian-like glycosylated proteins in insect cells.

 

Nucleic Acids
Product Benefit
Micro-to-Midi Total RNA Purification System Isolate the highest quality total RNA, from 100 cells to 200 mg of tissue.
Custom Oligonucleotides Quality customization from the leading provider of custom oligonucleotides gives you the best results every time.
0.17-1.77 Kb RNA Ladder For sizing single-stranded RNA in denaturing gels.
SuperScript™ III Reverse Transcriptase Generates the highest yield of cDNA during first-strand synthesis.
E. coli DNA Polymerase I Optimized polymerase is used in second-strand cDNA synthesis.
RNA UltraSense™ One-Step Quantitative RT-PCR System For amplification of RNA viruses and ultra-low abundance transcripts with SuperScript™ III RT and Platinum® Taq DNA Polymerase

 

Microarrays and Real-Time PCR
Product Benefit
SuperScript™ Indirect cDNA Array Labeling Kit Comes complete and optimized with all reagents (except fluorescent dyes).
Human Cot-1 DNA® Gold standard for blocking non-specific background during hybridization (human genes).
Platinum® Quantitative RT-PCR Thermoscript™ One-Step System This enzyme mix with optimized combination of high thermostability and RNase H- gives the highest yield for difficult RNA templates.
Custom LUX™ Fluorogenic Primers (for detection in RT-PCR or qPCR) Comparable to dual-labeled probes at half the cost.
SuperScript™ First-Strand Synthesis System for RT-PCR System is optimized for high-performance first-strand synthesis using SuperScript™ RT.

 

Protein Analysis
Product Benefit
ZOOM® IPGRunner™ 2D Gel Electrophoresis Cassettes Ready-to-use self-contained electrophoresis cassettes. Each cassette runs six strips, with two cassettes fitting in the ZOOM® IPGRunner™ unit.
ZOOM® IPGRunner™ Strips and Ampholytes Each ZOOM® strip is labeled with a unique identifying number, pH range, and orientation marks for easy usage.
NuPAGE® Bis-Tris ZOOM® Gels and Buffers Neutral pH Bis-Tris gels provide the sharpest spots with the least protein modification for the most accurate results in mass spectromity analysis of proteins.
SimplyBlue™ SafeStain A fast, sensitive, environmentally-safe redundant Coomassie® G-250 stain that detects nanogram levels of protein and is mass spectrometry-compatible.
SilverQuest™ Silver Staining Kit A fast, ultra-sensitive silver stain which is formulated to provide minimal protein modification for optimal mass spectrometry results.
WesternBreeze® Immunodetection Kits Optimized with everything you need for a successful western blot while reducing your transfer optimization time. Available for chromogenic and chemiluminescent detection.
MagicMark™ Western Protein Standard Allows for easy molecular weight estimation directly on western blots. Each band contains an IgG binding site so you can detect the standard with the same antibody-conjugates used to detect your sample.
Anti-Epitope Antibodies Provides purified mouse monoclonal antibodies which detect recombinant proteins containing the epitope tag.
Custom Antibody Services Create monospecific polyclonals against peptides or whole protein. We offer a wide range of antibody services, and with EvoTrack™ Online, you can manage your projects on the web easily and securely.
Custom Peptide Services Based on your specifications, we synthesize the peptide sequence of your choice in state-of-the art facilities. Several scales, purities and modifications available to ensure you get exactly what you want.

 

Software
Product Benefit
Vector NTI Advance™ and Vector NTI® Suite for Mac OS X The leading package for customized DNA and protein sequence analysis-customizable to meet your research and detection requirements at your fingertips.
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