Total RNA Extraction

Achieve RNA purification from a range of sample sizes and a wide variety of sample types including animal and plant tissue, yeast, bacteria, and blood using Ambion® products, PureLink™ spin columns, or TRIzol® organic extraction reagents.

Isolation of high quality, pure, and intact total RNA is a critical step in performing many fundamental downstream molecular biology experiments, including Northern blots, nuclease protection assays, RT-PCR, qRT-PCR and array analysis.

Which Total RNA Purification Kit is Right for You?

  Gold standard for highly pure, intact RNA High-quality RNA in less than 20 minutes High-throughput purification of RNA and DNA Complete, no purification system for qRT-PCR results
Recommendations Most Cited, Top Seller     Best for qPCR
Isolation method High purity organic extraction (requires alcohol precipitation) Fast, convenient silica column Scalable, flexible format with magnetic beads Chemical lysis (time to qRT-PCR results)
Prep time ~1 hr < 20 minutes ~45 minutes 10 minutes
Compatible sample types Most sample types, particularly more difficult to lyse Cells and tissues Cells, blood, plants Cells
Amount of starting material 100 mg of tissue or 107 cells (requires 1 mL reagent) Up to 200 mg tissue, 5x106 to 1x108 cells Up to 100 mg tissue, Up to 5x106 cells 1–100,000 cells
Reverse transcription reagents included       yes
qPCR Master mix included       yes
High throughput compatible   yes
(plate format available)		 yes yes
Price per prep range $1.40–$7.42 $4.50–$6.03 $3.00–$8.00 $9.62– $19.00
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Organic Extraction & Purification

Organic solvent extraction offers tried-and-true performance, and is ideal for the most stringent applications when combined with silica-membrane filtration or magnetic bead purification. TRIzol® Reagent offers:

  • Broadest sample type compatibility
  • Robust method
  • Good for difficult samples (e.g. high lipid tissue) and scalable sample input
  • Wide acceptance as the gold standard

 

 

 

 Learn more about TRIzol® Reagent

Magnetic Bead Purification

Magnetic particles offer solution-phase binding and washing kinetics to facilitate RNA purification and improved sample handling, including automation, ideal for higher throughput processing. MagMAX™ Magnetic Beads offer:

  • Flexible and scalable throughput
  • Amenable to automation for higher throughput
  • Better removal of inhibitors and low elution volume resulting in more concentrated RNA


 Learn more about MagMAX™ Magnetic Beads

Ambion® Brand RNA Essentials

Ambion® brand products, are designed to minimize nuclease contamination and ensure experimental success in less-than-ideal conditions. These products are manufactured under the most stringent quality specifications and include:

  • RNA purification kits
  • Specimen collection and RNA stabilization solutions
  • Nuclease inhibitors and decontaminants
  • Nuclease-free buffers and plastics
  • RNA ladders
  • Northern blot reagents

 

Learn more about Ambion® brand products

Silica-membrane Purification

Silica-membrane filters allow fast and easy RNA purification resulting in pure RNA that can be used in most applications. PureLink™ Purification columns offer:

 

  • A fast and easy method suitable for most downstream uses
  • Wide sample type compatibility
  • Familiar spin column format commonly used for low-med throughput

 

Learn more about PureLink™ Purification columns

Technical Resources

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