microRNA & small RNA Isolation
 | MicroRNAs (miRNAs) are small, highly conserved RNA molecules that, like small interfering RNAs (siRNAs), modulate the expression of their cognate mRNA molecules by base-pairing with them and preventing expression through a variety of mechanisms. They have been shown to act as key regulators of development, cell proliferation, differentiation, and the cell cycle. There are several other classes of small noncoding RNAs as well, including small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs), and piwi-interacting RNA (piRNA). These varied species of RNA have tremendous impact on biological processes, yet most traditional glass-fiber filter RNA isolation methods were developed to recover only mRNA and are not very efficient at recovering these smaller RNAs. Life Technologies offers several kits for the quantitative recovery of small RNAs from a variety of sample types. |
Which microRNA & small RNA Isolation Kit if Right for You?
| Purification for enrichment of specific small RNAs | Rapid purification for enrichment of small RNAs only | 30 min isolation from most samples | HTP, pure, concentrated microRNAs | Complete kit, sample-to-qPCR from cells | |||||
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| TaqMan® ABC miRNA Purification Kit | |||||||||
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| RNA types isolated | Small RNAs only | Small RNAs only | Small & large RNA molecules | Small & large RNA molecules | Small & large RNA molecules | ||||
| Prep time | 75 minutes | 20 minutes | 30 minutes | <1 hour | 10 minutes | ||||
| Compatible sample types | Fresh or frozen tissue, blood, cells | Fresh or frozen tissue, tissue stored in RNAlater, cells | Most sample types | Fresh or frozen tissue, tissue stored in RNAlater, cells | Cells | ||||
| Isolation method | 10-50 μL blood-related samples; 5 x 104 cultured cells or 1-10 mg tissue | Fast, convenient silica column | Highest purity and convenience; includes both organic extraction and silica column | Scalable, flexible format for highest purity; includes organic extraction and magnetic beads | Cells-to-CT™ direct lysis, no purification required | ||||
| Reverse transcription reagents included | No | No | No | No | TaqMan® MicroRNA Reverse Transcription Kit | ||||
| qPCR Master mix included | No | No | No | No | TaqMan® | ||||
| Kit size | 40 Preps | 25 preps | 40 preps | 96 preps | 100 lysis reactions - plus - reagents for 200 qRT-PCR assays |
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| Kit Price | $340.00 | $175.88 | $225-266.00 | $373.00 | $1,356.00 | ||||
| Order Now | Order Now | Order Now | Order Now | Order Now |
Technical Resources
External Web Resources
microRNA Video
Ambion Publications
- Johnson CD, Esquela-Kerscher A, Stefani G, Byrom M, Kelnar K, Ovcharenko D, Wilson M, Wang X, Shelton J, Shingara J, Chin L, Brown D, Slack FJ. The let-7 MicroRNA Represses Cell Proliferation Pathways in Human Cells. Cancer Res. 2007 Aug 15;67(16):7713-22. [Abstract]
- Shingara J, Keiger K, Shelton J, Laosinchai-Wolf W, Powers P, Conrad R, Brown D, Labourier E. An optimized isolation and labeling platform for accurate microRNA expression profiling.RNA. 2005 Jul 25; [Epub ahead of print] [Abstract]
- Johnson SM, Grosshans H, Shingara J, Byrom M, Jarvis R, Cheng A, Labourier E, Reinert KL, Brown D, Slack FJ. RAS is regulated by the let-7 microRNA family. Cell. 2005 Mar 11;120(5):635-47. [Abstract]
- Cheng AM, Byrom MW, Shelton J, Ford LP. Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis. Nucleic Acids Res. 2005 Mar 1;33(4):1290-7. [Abstract]
